影响因子：17.97

DOI码：10.1016/j.molcel.2020.06.001

发表刊物：Molecular Cell (B刊)

摘要：Somatic cell nuclear transfer (SCNT) can reprogram a somatic nucleus to a totipotent state. However, the re-organization of 3D chromatin structure in this process remains poorly understood. Using low-input Hi-C, we revealed that, during SCNT, the transferred nucleus first enters a mitotic-like state (premature chromatin condensation). Unlike fertilized embryos, SCNT embryos show stronger topologically associating domains (TADs) at the 1-cell stage. TADs become weaker at the 2-cell stage, followed by gradual consolidation. Compartments A/B are markedly weak in 1-cell SCNT embryos and become increasingly strengthened afterward. By the 8-cell stage, somatic chromatin architecture is largely reset to embryonic patterns. Unexpectedly, we found cohesin represses minor zygotic genome activation (ZGA) genes (2-cell-specific genes) in pluripotent and differentiated cells, and pre-depleting cohesin in donor cells facilitates minor ZGA and SCNT. These data reveal multi-step reprogramming of 3D chromatin architecture during SCNT and support dual roles of cohesin in TAD formation and minor ZGA repression.

合写作者：Sun QR,Liu X,Wang LY,Xiong WJ,Wang QJ,Rhodes JDP,Xu K,Li LJ,Lin ZL,Yu G,Xia WK,Huang B,Du ZH,Yao Y,Nasmyth KA,Klose RJ

第一作者：Wang Y#,Zheng H#,Wu DY#,Zhang K#

论文类型：期刊论文

通讯作者：Miao YL*,Xie W*

卷号：79

期号：2

页面范围：234-250.e9.

是否译文：否

发表时间：2020-07-16

收录刊物：SCI

发布期刊链接：https://www.cell.com/molecular-cell/fulltext/S1097-2765(20)30386-5