影响因子：5.843

DOI码：10.1242/dev.158261

发表刊物：Development (C刊)

摘要：Aberrant epigenetic reprogramming often results in developmental defects in somatic cell nuclear transfer (SCNT) embryos during embryonic genome activation (EGA). Bovine eight-cell SCNT embryos exhibit global hypermethylation of histone H3 lysine 9 tri- and di-methylation (H3K9me3/2), but the intrinsic reason for this remains elusive. Here, we provide evidence that two H3K9 demethylase genes, lysine-specific demethylase 4D (KDM4D) and 4E (KDM4E), are related to active H3K9me3/2 demethylation in in vitro fertilized (IVF) embryos and are deficiently expressed in cloned embryos at the time of EGA. Moreover, KDM4E plays a more crucial role in IVF and SCNT embryonic development, and overexpression of KDM4E can restore the global transcriptome, improve blastocyst formation and increase the cloning efficiency of SCNT embryos. Our results thereby indicate that KDM4E can function as a crucial epigenetic regulator of EGA and as an internal defective factor responsible for persistent H3K9me3/2 barriers to SCNT-mediated reprogramming. Furthermore, we show that interactions between RNA and KDM4E are essential for H3K9 demethylation during EGA. These observations advance the understanding of incomplete nuclear reprogramming and are of great importance for transgenic cattle procreation.

合写作者：Wang YZ,Gao YP,Su JM,Zhang JC,Xing XP,Zhou C,Yao KZ,An QL

第一作者：Liu X#

论文类型：期刊论文

通讯作者：Zhang Y*

卷号：145

期号：4

页面范围：dev158261

是否译文：否

发表时间：2018-02-16

收录刊物：SCI

发布期刊链接：https://journals.biologists.com/dev/article/145/4/dev158261/48605/