Impact Factor:4.125

DOI number:10.1074/jbc.M117.796771

Journal:Journal of Biological Chemistry (C刊)

Abstract:Somatic cell nuclear transfer (SCNT)-mediated reprogramming is a rapid, efficient, and sophisticated process that reprograms differentiated somatic cells to a pluripotent state. However, many factors in this elaborate reprogramming process remain largely unknown. Here, we report that the microRNA (miR) miR-125b is an important component of SCNT-mediated reprogramming. Luciferase reporter assay, quantitative PCR, and Western blotting demonstrated that miR-125b directly binds the 3′-untranslated region of SUV39H1, encoding the histone-lysine N-methyltransferase SUV39H1, to down-regulate histone H3 lysine-9 tri-methylation (H3K9me3) in SCNT embryos. Furthermore, the miR-125b/SUV39H1 interaction induced loss of SUV39H1-mediated H3K9me3, caused heterochromatin relaxation, and promoted the development of SCNT embryos. Transcriptome analyses of SCNT blastomeres indicated that HNF1 homeobox B (HNF1B), a gene encoding a transcription factor downstream of and controlled by the miR-125b/SUV39H1 axis, is important for conferring developmental competence on preimplantation embryos. We conclude that miR-125b promotes SCNT-mediated nuclear reprogramming by targeting SUV39H1 to decrease the deposition of repressive H3K9me3 modifications.

Co-author:Zhou C,Liu X,Ma XN,Wang MY,Su JM,Liu J

First Author:Zhang JC#,Qu PX#

Indexed by:Journal paper

Correspondence Author:Wang YS*,Zhang Y*

Volume:292

Issue:38

Page Number:15916-15926

Translation or Not:no

Date of Publication:2017-08-09

Included Journals:SCI

Links to published journals:https://www.jbc.org/article/S0021-9258(20)34056-4/fulltext#articleInformation