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刘鑫

Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
Name (Simplified Chinese):刘鑫
Name (Pinyin):Liu Xin
E-Mail:
School/Department:动物科学技术学院、动物医学院
Business Address:第四综合楼E615
Professional Title:Associate researcher
Alma Mater:西北农林科技大学
Teacher College:College of Animal Sciences & Technology / College of Veterinary Medicine
Discipline:Animal Genetic Breeding and Reproduction    
Other Contact Information:

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Paper Publications
H3K9 demethylase KDM4E is an epigenetic regulator for bovine embryonic development and a defective factor for nuclear reprogramming
Release time:2021-11-23    Hits:

Impact Factor:5.843

DOI number:10.1242/dev.158261

Journal:Development (C刊)

Abstract:Aberrant epigenetic reprogramming often results in developmental defects in somatic cell nuclear transfer (SCNT) embryos during embryonic genome activation (EGA). Bovine eight-cell SCNT embryos exhibit global hypermethylation of histone H3 lysine 9 tri- and di-methylation (H3K9me3/2), but the intrinsic reason for this remains elusive. Here, we provide evidence that two H3K9 demethylase genes, lysine-specific demethylase 4D (KDM4D) and 4E (KDM4E), are related to active H3K9me3/2 demethylation in in vitro fertilized (IVF) embryos and are deficiently expressed in cloned embryos at the time of EGA. Moreover, KDM4E plays a more crucial role in IVF and SCNT embryonic development, and overexpression of KDM4E can restore the global transcriptome, improve blastocyst formation and increase the cloning efficiency of SCNT embryos. Our results thereby indicate that KDM4E can function as a crucial epigenetic regulator of EGA and as an internal defective factor responsible for persistent H3K9me3/2 barriers to SCNT-mediated reprogramming. Furthermore, we show that interactions between RNA and KDM4E are essential for H3K9 demethylation during EGA. These observations advance the understanding of incomplete nuclear reprogramming and are of great importance for transgenic cattle procreation.

Co-author:Wang YZ,Gao YP,Su JM,Zhang JC,Xing XP,Zhou C,Yao KZ,An QL

First Author:Liu X#

Indexed by:Journal paper

Correspondence Author:Zhang Y*

Volume:145

Issue:4

Page Number:dev158261

Translation or Not:no

Date of Publication:2018-02-16

Included Journals:SCI

Links to published journals:https://journals.biologists.com/dev/article/145/4/dev158261/48605/