Impact Factor:10.384

DOI number:10.1007/s11427-022-2349-9

Journal:SCIENCE CHINA Life Sciences (C刊)

Abstract:Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos. Current studies have illustrated several pioneer factors an important agricultural animal, what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos are not clear. By a low-input DNase-seq (liDNase-seq), we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development. Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos. Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos, whereas KLF4 overexpression promoted the chromatin openness in porcine blastocysts. Furthermore, KLF4 deficiency resulted in mitochondrial malfunction and developmental failure of porcine embryos. In addition, we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation, whereas knockdown of KLF4 disrupted this process. Taken together, our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development.

Co-author:Zhou K,Wang TT,Li Q,Zhang JJ,Wu LH,Xie YL,Hu TT,Yang SC,Guan JQ,Chu XY,Shi JJ,Zhang X

First Author:Zhu W#,Bu GW#,Hu RF#,Zhang JX#,Qiao LY#

Indexed by:Journal paper

Correspondence Author:Lu FL*,Liu X*,Miao YL*

Volume:Online

Page Number:1-31

Translation or Not:no

Date of Publication:2023-05-05

Included Journals:SCI

Links to published journals:https://www.sciengine.com/SCLS/doi/10.1007/s11427-022-2349-9