Impact Factor:17.521

DOI number:10.1002/advs.202200057

Journal:Advanced Science (A刊)

Abstract:Early embryos undergo extensive epigenetic reprogramming to achieve gamete-to-embryo transition, which involves the loading and removal of histone variant H2A.Z on chromatin. However, how does H2A.Z regulate gene expression and histone modifications during preimplantation development remains unrevealed. Here, by using ultra-low-input native chromatin immunoprecipitation and sequencing, the genome-wide distribution of H2A.Z is delineated in mouse oocytes and early embryos. These landscapes indicate that paternal H2A.Z is removed upon fertilization, followed by unbiased accumulation on parental genomes during zygotic genome activation. Remarkably, H2A.Z exhibits hierarchical accumulation as different peak types at promoters: promoters with double H2A.Z peaks are co-localized with H3K4me3 and indicate transcriptional activation; promoters with a single H2A.Z peak are more likely to occupy bivalent marks (H3K4me3+H3K27me3) and indicate development gene suppression; promoters with no H2A.Z accumulation exhibit persisting gene silencing in early embryos. Moreover, H2A.Z depletion changes the enrichment of histone modifications and RNA polymerase II binding at promoters, resulting in abnormal gene expression and developmental arrest during lineage commitment. Furthermore, similar transcription and accumulation patterns between mouse and porcine embryos indicate that a dual role of H2A.Z in regulating the epigenome required for proper gene expression is conserved during mammalian preimplantation development.

Co-author:Zhou JL,Zhu W,Bu GW,He HN,Sun QR,Yu ZS,Xiong WJ,Wang LY,Wu DY,Dou CL,Yu LT,Zhou K,Wang SK,Fan ZG,Wang TT,Hu RF,Hu TT,Zhang X

First Author:Liu X#,Zhang JJ#

Indexed by:Journal paper

Correspondence Author:Miao YL*

Volume:Online

Page Number:1-18

Translation or Not:no

Date of Publication:2022-05-19

Included Journals:SCI

Links to published journals:https://onlinelibrary.wiley.com/doi/10.1002/advs.202200057