访问量:   最后更新时间:--

刘鑫

博士生导师
硕士生导师
教师姓名:刘鑫
教师拼音名称:Liu Xin
电子邮箱:
办公地点:第四综合楼E615
职称:副研究员
毕业院校:西北农林科技大学
所属院系:动物科学技术学院、动物医学院
学科:动物遗传育种与繁殖    
其他联系方式

暂无内容

论文成果
TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos
发布时间:2021-11-11    点击次数:

影响因子:7.765

DOI码:10.1016/j.stemcr.2021.09.012

发表刊物:Stem Cell Reports (C刊)

摘要:Pig cloning by somatic cell nuclear transfer (SCNT) frequently undergoes incomplete epigenetic remodeling during the maternal-to-zygotic transition, which leads to a significant embryonic loss before implantation. Here, we generated the first genome-wide landscapes of histone methylation in pig SCNT embryos. Excessive H3K9me3 and H3K27me3, but not H3K4me3, were observed in the genomic regions with unfaithful embryonic genome activation and donor-cell-specific gene silencing. A combination of H3K9 demethylase KDM4A and GSK126, an inhibitor of H3K27me3 writer, were able to remove these epigenetic barriers and restore the global transcriptome in SCNT embryos. More importantly, thymine DNA glycosylase (TDG) was defined as a pig-specific epigenetic regulator for nuclear reprogramming, which was not reactivated by H3K9me3 and H3K27me3 removal. Both combined treatment and transient TDG overexpression promoted DNA demethylation and enhanced the blastocyst-forming rates of SCNT embryos, thus offering valuable methods to increase the cloning efficiency of genome-edited pigs for agricultural and biomedical purposes.

合写作者:Zhou JL,Li ZK,Bu GW,Zhang JJ,Yin SY,Wu DY,Dou CL,Xu T,He HN,Zhu W,Yu LT,Liu ZT,Zhang X

第一作者:Liu X#,Chen L#,Wang T#

论文类型:期刊论文

通讯作者:Miao YL*,Chen ZX*

卷号:16

期号:11

页面范围:2674-2689

是否译文:

发表时间:2021-10-21

收录刊物:SCI

发布期刊链接:https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(21)00489-6