影响因子：10.384

DOI码：10.1007/s11427-022-2349-9

发表刊物：SCIENCE CHINA Life Sciences (C刊)

摘要：Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos. Current studies have illustrated several pioneer factors an important agricultural animal, what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos are not clear. By a low-input DNase-seq (liDNase-seq), we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development. Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos. Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos, whereas KLF4 overexpression promoted the chromatin openness in porcine blastocysts. Furthermore, KLF4 deficiency resulted in mitochondrial malfunction and developmental failure of porcine embryos. In addition, we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation, whereas knockdown of KLF4 disrupted this process. Taken together, our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development.

合写作者：Zhou K,Wang TT,Li Q,Zhang JJ,Wu LH,Xie YL,Hu TT,Yang SC,Guan JQ,Chu XY,Shi JJ,Zhang X

第一作者：Zhu W#,Bu GW#,Hu RF#,Zhang JX#,Qiao LY#

论文类型：期刊论文

通讯作者：Lu FL*,Liu X*,Miao YL*

卷号：Online

页面范围：1-31

是否译文：否

发表时间：2023-05-05

收录刊物：SCI

发布期刊链接：https://www.sciengine.com/SCLS/doi/10.1007/s11427-022-2349-9