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刘鑫

博士生导师
硕士生导师
教师姓名:刘鑫
教师拼音名称:Liu Xin
电子邮箱:
办公地点:第四综合楼E615
职称:副研究员
毕业院校:西北农林科技大学
所属院系:动物科学技术学院、动物医学院
学科:动物遗传育种与繁殖    
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论文成果
Hierarchical accumulation of histone variant H2A.Z regulates transcriptional states and histone modifications in early mammalian embryos
发布时间:2022-05-29    点击次数:

影响因子:17.521

DOI码:10.1002/advs.202200057

发表刊物:Advanced Science (A刊)

摘要:Early embryos undergo extensive epigenetic reprogramming to achieve gamete-to-embryo transition, which involves the loading and removal of histone variant H2A.Z on chromatin. However, how does H2A.Z regulate gene expression and histone modifications during preimplantation development remains unrevealed. Here, by using ultra-low-input native chromatin immunoprecipitation and sequencing, the genome-wide distribution of H2A.Z is delineated in mouse oocytes and early embryos. These landscapes indicate that paternal H2A.Z is removed upon fertilization, followed by unbiased accumulation on parental genomes during zygotic genome activation. Remarkably, H2A.Z exhibits hierarchical accumulation as different peak types at promoters: promoters with double H2A.Z peaks are co-localized with H3K4me3 and indicate transcriptional activation; promoters with a single H2A.Z peak are more likely to occupy bivalent marks (H3K4me3+H3K27me3) and indicate development gene suppression; promoters with no H2A.Z accumulation exhibit persisting gene silencing in early embryos. Moreover, H2A.Z depletion changes the enrichment of histone modifications and RNA polymerase II binding at promoters, resulting in abnormal gene expression and developmental arrest during lineage commitment. Furthermore, similar transcription and accumulation patterns between mouse and porcine embryos indicate that a dual role of H2A.Z in regulating the epigenome required for proper gene expression is conserved during mammalian preimplantation development.

合写作者:Zhou JL,Zhu W,Bu GW,He HN,Sun QR,Yu ZS,Xiong WJ,Wang LY,Wu DY,Dou CL,Yu LT,Zhou K,Wang SK,Fan ZG,Wang TT,Hu RF,Hu TT,Zhang X

第一作者:Liu X#,Zhang JJ#

论文类型:期刊论文

通讯作者:Miao YL*

卷号:Online

页面范围:1-18

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发表时间:2022-05-19

收录刊物:SCI

发布期刊链接:https://onlinelibrary.wiley.com/doi/10.1002/advs.202200057