谢胜松，中共党员，华中农业大学教授。2019年-2020年国际家畜研究所（ILRI）访问学者。华中农业大学学士、农学硕士，中国科学院大学理学博士。2013年4月至2014年11月为中国科学院上海生命科学研究院助理研究员。现华中农业大学动科动医学院基础兽医系，农业动物遗传育种与繁殖教育部重点实验室固定成员，创新人才推进计划重点领域“猪基因组与育种创新团队”核心成员之一。长期坚持在科研、教学一线，致力于科学研究、人才培养和平台建设。研究方向为猪基因编辑育种，致力于利用CRISPR技术开展猪功能基因筛选与核酸即时检测研究。率先搭建了猪全基因组CRISPR文库高通量筛选平台，鉴定到一批参与病毒增殖的关键基因；创制了基因编辑猪育种新材料，研发了RAVI-CRISPR核酸裸眼可视化检测新技术。相关研究成果发表于Nat Commun，PLoS Pathog，Bioinformatics等行业主流期刊，其中sgRNAcas9软件文章被引300余次。主持国家自然基金青年、面上项目，参与科技重大专项和重点研发子课题等。任Frontiers in Genetics客座编辑；发表SCI论文16余篇，授权国家发明专利9件，软件著作权2件，申请美国专利1件，其中有1项发明专利已转化应用。受邀参加第二十一次全国动物遗传育种学术讨论会并荣获吴常信动物遗传育种优秀论文奖。2020年获教育部科技进步一等奖（排名 9/13）。

已发表的论文：

1.Xie S#, Tao D#, Fu Y#, Xu B#, Tang Y, Steinaa L, Hemmink JD, Pan W, Huang X, Nie X, Zhao C, Ruan J, Zhang Y, Han J, Fu L, Ma Y, Li X*, Liu X*, Zhao S*. Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network. ACS Synth Biol. 2022 Jan 21;11(1):383-396. doi: 10.1021/acssynbio.1c00474. Epub 2021 Dec 23.

2.Sun L#, Zhao C#, Fu Z, Fu Y, Su Z, Li Y, Zhou Y, Tan Y, Li J, Xiang Y, Nie X, Zhang J, Liu F, Zhao S, Xie S*, Peng G*. Genome-scale CRISPR screen identifies TMEM41B as a multi-function host factor required for coronavirus replication. PLoS Pathog. 2021 Dec 6;17(12):e1010113. doi: 10.1371/journal.ppat.1010113. Online ahead of print.

3.Liu S, Tao D, Liao Y, Yang Y, Sun S, Zhao Y, Yang P, Tang Y, Chen B, Liu Y, Xie S*, Tang Z*. Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus. ACS Synth Biol. 2021 Oct 15;10(10):2499-2507. doi: 10.1021/acssynbio.1c00103. Epub 2021 Sep 20.

4.Zhang J, Khazalwa EM, Abkallo HM, Zhou Y, Nie X, Ruan J, Zhao C, Wang J, Xu J, Li X, Zhao S, Zuo E*, Steinaa L*, Xie S*. The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research. J Genet Genomics. 2021 May 20;48(5):347-360. doi: 10.1016/j.jgg.2021.03.015. Epub 2021 May 11.

5.Han X, Gao Y, Li G, Xiong Y, Zhao C, Ruan J, Ma Y, Li X, Li C, Zhao S, Xie S*. Enhancing the antibacterial activities of sow milk via site-specific knock-in of a lactoferrin gene in pigs using CRISPR/Cas9 technology. Cell Biosci. 2020 Nov 19;10(1):133. doi: 10.1186/s13578-020-00496-y.

6.Zhao C#, Liu H#, Xiao T#, Wang Z, Nie X, Li X, Qian P, Qin L, Han X, Zhang J, Ruan J, Zhu M, Miao YL, Zuo B, Yang K, Xie S*, Zhao S*. CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication. Nat Commun. 2020 Oct 14;11(1):5178. doi: 10.1038/s41467-020-18936-1.

7.Tao D#, Liu J#, Nie X, Xu B, Tran-Thi TN, Niu L, Liu X, Ruan J, Lan X, Peng G, Sun L, Ma Y, Li X, Li C, Zhao S, Xie S*. Application of CRISPR-Cas12a Enhanced Fluorescence Assay Coupled with Nucleic Acid Amplification for the Sensitive Detection of African Swine Fever Virus. ACS Synth Biol. 2020 Sep 18;9(9):2339-2350. doi: 10.1021/acssynbio.0c00057. Epub 2020 Aug 31. PMID: 32786346

8.Zhao C#, Wang Y#, Nie X, Han X, Liu H, Li G, Yang G, Ruan J, Ma Y, Li X, Cheng H, Zhao S, Fang Y*, Xie S*. Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity. Int J Biol Sci. 2019 Oct 3;15(12):2641-2653. doi: 10.7150/ijbs.37152. eCollection 2019.

9.Xie S#, Zhu Q#, Qu W#, Xu Z, Liu X, Li X, Li S, Ma W, Miao Y, Zhang L, Du X, Dong W, Li H, Zhao C, Wang Y, Fang Y*, Zhao S*. sRNAPrimerDB: comprehensive primer design and search web service for small non-coding RNAs. Bioinformatics. 2019 May 1;35(9):1566-1572. doi: 10.1093/bioinformatics/bty852.

10.Ahmad HI, Ahmad MJ, Asif AR, Adnan M, Iqbal MK, Mehmood K, Muhammad SA, Bhuiyan AA, Elokil A, Du X, Zhao C, Liu X*, Xie S*. A Review of CRISPR-Based Genome Editing: Survival, Evolution and Challenges. Curr Issues Mol Biol. 2018;28:47-68. doi: 10.21775/cimb.028.047. Epub 2018 Feb 11.

11.Zhao C#, Zheng X#, Qu W#, Li G, Li X, Miao YL, Han X, Liu X, Li Z, Ma Y, Shao Q, Li H, Sun F*, Xie S*, Zhao S*. CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif. Int J Biol Sci. 2017 Nov 1;13(12):1470-1478. doi: 10.7150/ijbs.21312. eCollection 2017.

12.Xu J, Yao G, Ru Y, Xie S*. Expression of tamoxifen-inducible CRE recombinase in Lcn5-CreERT2 transgenic mouse caput epididymis. Mol Reprod Dev. 2017 Mar;84(3):257-264. doi: 10.1002/mrd.22772. Epub 2017 Mar 16.

13.Xie S, Shen B, Zhang C, Huang X, Zhang Y. sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One. 2014 Jun 23;9(6):e100448. doi: 10.1371/journal.pone.0100448. eCollection 2014.

14.Xie S#, Xu J#, Ma W, Liu Q, Han J, Yao G, Huang X*, Zhang Y*. Lcn5 promoter directs the region-specific expression of cre recombinase in caput epididymidis of transgenic mice. Biol Reprod. 2013 Mar 21;88(3):71. doi: 10.1095/biolreprod.112.104034. Print 2013 Mar.

15.Xie S#, Li X#, Liu T, Cao J, Zhong Q, Zhao S. Discovery of porcine microRNAs in multiple tissues by a Solexa deep sequencing approach.PLoS One. 2011 Jan 25;6(1):e16235. doi: 10.1371/journal.pone.0016235.

16.Xie S, Huang T, Shen Y, Li X, Zhang X, Zhu M, Qin H, Zhao S*. Identification and characterization of microRNAs from porcine skeletal muscle. Anim Genet. 2010 Apr;41(2):179-90. doi: 10.1111/j.1365-2052.2009.01991.x. Epub 2009 Nov 26.