李林，男，1965年出生，理学博士，教授，博士生导师。华中农业大学生命科学技术学院教师，农业微生物学资源发掘与利用全国全国重点实验室PI。1999年毕业于华中农业大学生命科学技术学院微生物学专业，获理学博士学位。先后于2000-2002、2003-2004年和2009先后赴韩国浦项工科大学、德国Max-Planck-Institut fuer InfektionsBiologie研究所和美国加州大学戴维斯分校分别从事博士后（或访问学者）研究工作。目前主要从事微生物生物技术方面的研究工作。曾任中国微生物学会基础微生物专业委员会副主任。Springer/Elsevier期刊、Front Microbiol和IJMS等期刊编辑。国家科技进步奖函评专家，以及国家科技部、国家自然科学基金委员会、教育部、湖北省、江苏省、江西省和福建省等多个省部级科技计划、科技成果和人才项目评审专家。先后主持国家863项目、国家973项目课题和国家自然科学基金项目等科研项目20余项。

科研方向：

以假单胞菌、芽孢杆菌、大肠杆菌和谷氨酸棒杆菌等原核细菌和酵母菌为对象，主要进行环境降解、底盘细胞改造、功能元件研发与适配性研究、基因表达活性调控、作用机制与应用技术基础等方面的研究工作。目前主要科研方向为：

（1）假单胞菌降解环境难降解和有毒化合物的功能基因、降解活性、作用途径与机制；

（2）运用CRISPR-Cas9/CRISPR-Cas12f1、CRISPR-RNA等基因编辑工具进行假单胞菌、芽孢杆菌、大肠杆菌和谷氨酸棒杆菌基因组简约编辑，规模性删除基因组冗余基因和改造细菌底盘细胞；

（3）体内连续进化并联合体外进化策略，以理性设计、半理性设计和构建自组装人工多酶复合体等生物技术策略实现对不同酶蛋白的分子改造，以大幅提升酶催化活性与酶底物适配性；

（4）针对细菌和酵母细胞工厂不同元件的适配性研究。已在假单胞菌、大肠杆菌和酵母菌成功发掘不同强度的系列启动子、RBS、群体感应系统单元和菌体自杀系统单元，以适应于不同使用目的需要。

学术成绩：

长期以来主要围绕假单胞菌环境降解、致病与杀虫相关的功能基因、表达调控、作用机制与应用技术基础等方面进行了一系列研究工作，发表SCI论文80余篇，其它学术论文30余篇，参与撰写英文学术专著2部，中文学术专著2部，获批国家发明专7项、申报中4项。获得湖北省技术发明一等奖（第6）、农业部科技进步三等奖（第6）和武汉市科技进步二等奖（第5）等奖励。

主持的主要科研项目

[1]     武汉市科技重大专项“功能营养素合成生物绿色制造关键技术攻关”子课题“高产精氨酸菌株构建”（2023020302020708），2023/10-2025/10

[2]     国家自然科学基金，CRISPR-RNA整合功能基因元件的工程假单胞菌矿化双酚EDCs耦合代谢机制与微环境应用效果（32170124），2022/01-2025/12

[3]     国家自然科学基金，利用II型CRISPR-Cas9系统改造锰氧化假单胞菌T34及用于生物模板法构建高势能电极材料的研究（31770108），2018/01-2021/12

[4]     国家自然科学基金，丁香假单胞菌GntR/HutC类转录调控因子InpR调控网络解析与调控冰核基因分子机制研究（31570123），2016/01-2019/123.

[5]     国家重点基础研究计划（973计划）课题，细菌侵染线虫的分子机制（2013CB127504），2013/01-2017/08

[6]     湖北省公益性科技研究项目，广谱高效的苏云金芽孢杆菌纳米工程菌制剂的研制与应用技术研究（2012DBA10001），2013/01-2014/125.

[7]     国家自然科学基金，土壤细菌生物氧化Mn(II)的分子机制研究（31270158），2013/01-2016/12

[8]     国家自然科学基金，丁香假单胞菌冰核蛋白跨膜分泌效应分子的研究（31070111），2011/01-2013/12

[9]     国家自然科学基金重点项目（课题），土壤中锰氧化物的生物形成及对物质形成和转化的影响（40830527），2009/ 01－2012/ 12

[10] 国家高技术研究发展计划（863计划），基于组学分析和代谢工程技术分析的苏云金杆菌高毒力菌种的选育（2008AA02Z112），2008/01-2010/12

[11] 国家自然科学基金，用芽孢杆菌自溶素构建细菌表面展示体系及锚定机制的研究（30670054），2007/01-2009/12

[12] 国家自然科学基金，用冰晶核蛋白构建假单胞菌细胞表面展示体系及性能研究（30370026），2004/01-2006/12

部分学术论著 (*通讯作者)

1. Xiaowen Sun, Xue Zhang, Zhi Li, Tan Wang, Jie Zeng, Yongxuan Liu, Zhe Li, Lin Li*. Efficient remediation of di-(2-ethylhexyl) phthalate and plant-growth promotion with the application of a phosphate-solubilizing compound microbial agent. Science of the Total Environment 2024, 926: 171904.

2. Xun Yu, Yubo Yan, Jie Zeng, Yongxuan Liu, Xiaowen Sun, Zhiyong Wang, Lin Li*. T6SS nuclease effectors in Pseudomonas syringae act as potent antimicrobials in interbacterial competition.  Journal of Bacteriology 2024, 206(6): e0027323.

3. Xiaowen Sun, Fan Xiao, Yuwei Su, Zhi Li, Xun Yu, Rebecca E. Parales*, Lin Li*. Cyclic di-GMP incorporates the transcriptional factor FleQ03 in Pseudomonas syringae MB03 to elicit biofilm-dependent resistance in response to Caenorhabditis elegans predation. Journal of Invertebrate Pathology 2024, 207: 108189.

4. Xiaowen Sun, Zhe Li, Jiaoqing Li, Zhi Li, Yini Ma, Zhicheng Zhou, Yongxuan Liu, Jie Zeng, Liangzheng Xu, Lin Li*. Dynamic composting actuated by a Caldibacillus thermoamylovorans isolate enables biodecomposability and reusability of Cinnamomum camphora garden wastes. Bioresource Technology 2023, 376: 128852.
   

5. Yongxuan Liu, Jiaoqing Li, Jie Zeng, Xun Yu, Xiaowen Sun, Zhicheng Zhou, Jingjing Xu, Liangzheng Xu, Lin Li*. Complete oxidative degradation of diclofenac via coupling free radicals and oxygenases of a micro/nanostructured biogenic Mn oxide composite from engineered Pseudomonas sp. MB04R-2. Journal of Hazardous Materials 2023, 456: 131657.

6. Asmaa Sh. M. Daoush, Mohamed H. Hendawey, Rabaa Yaseen, Ahmed S. M. El-Nuby, Tarek M. Bedair, Khairiah Mubarak Alwutayd, Nawal Al-Hoshani, Ahmed Shaaban, Anum Bashir, Lin Li*. Effect of biosynthesized nanoselenium on controlling tomato root-knot nematode Meloidogyne incognita. Agronomy 2023, 13: 1668.

7. Xun Yu, Cui Meng, Xiaocheng Tan, Yuwei Su, Zhiping Cao, Tzann-Shun Hwang, Lin Li*. RsmA3 modulates RpoS through the RetS-Gac-Rsm signaling pathway in response to H₂O₂ stress in the phytopathogen Pseudomonas syringae. Environmental Microbiology 2022, 24: 4755-4770.

8. Muhammad Ali, Tong Gu, Xun Yu, Anum Bashir, Zhiyong Wang, Xiaowen Sun, Naeem Mahmood Ashraf, Lin Li*. Identification of the genes of the plant pathogen Pseudomonas syringae MB03 required for the nematicidal activity against Caenorhabditis elegans through an integrated approach. Frontiers in Microbiology 2022, 13: 826962.

9. Tan Wang, Kexin Yang, Qingyun Ma, Xu Jiang, Yiqing Zhou, Delong Kong, Zhiye Wang, Rebecca E. Parales, Lin Li*, Xin Zhao*, Zhiyong Ruan*. Rhizosphere microbial community diversity and function analysis of cut chrysanthemum during continuous monocropping. Frontiers in Microbiology 2022, 13: 801546.

10. Anum Bashir, Huafu Yu, Abdul Manan, Lin Li*. Nematicidal effects of a peptidase (NlpC/P60) from the phytopathogen Pseudomonas syringae MB03 on the model nematode Caenorhabditis elegans and plant-parasitic Meloidogyne incognita. Molecular Biology Reports 2022, 2022, 49: 6313-6324.

11. Tong Gu, Zhenghu Tong, Xue Zhang, Zhiyong Wang, Zhen Zhang, Tzann-Shun Hwang*, Lin Li*. Carbon metabolism of a soilborne Mn(II)-oxidizing Escherichia coli isolate implicated as a pronounced modulator of bacterial Mn oxidation. International Journal of Molecular Sciences 2022, 23: 5951.

12. Jiaoqing Li, Meijuan Ding, Xiaowen Sun, Zhe Li, Liangzheng Xu*, Lin Li*. Characterization of nematicidal activity and nematode-toxic metabolites of a soilborne Brevundimonas bullata isolate. Pathogens 2022, 11, 708.

13. Jin Liu, Tong Gu, Li Li, Lin Li*. Synthesis of MnO/C/NiO-doped porous multiphasic composites for lithium-ion batteries by biomineralized Mn oxides from engineered Pseudomonas putida cells. Nanomaterials 2021, 11: 361.

14. Xiaowen Su, Run Zhan, Meijuan Ding, Yongxuan Liu, Lin Li*. Biocontrol of the root-knot nematode Meloidogyne incognita by a nematicidal bacterium Pseudomonas simiae MB751 with cyclic dipeptide. Pest Management Science 2021, 77: 4365-4374.

15. Jin Liu, Tong Gu, Xiaowen Sun, Li Li, Fan Xiao, Zhiyong Wang, Lin Li*. Synthesis of MnO/C/Co₃O₄ nanocomposites by a Mn²⁺-oxidizing bacterium as a biotemplate for lithium-ion batteries. Science and Technology of Advanced Materials 2021, 22(1): 429-440.

16. Li Li, Jin Liu, Jie Zeng, Jiaoqing Li , Yongxuan Liu, Xiaowen Sun, Liangzheng Xu*, Lin Li*. Complete degradation and detoxification of ciprofloxacin by a Micro-/nanostructured biogenic Mn oxide composite from a highly active Mn²⁺-oxidizing Pseudomonas strain. Nanomaterials 2021, 11: 1660.

17. Anum Bashir, Yu Sun, Xun Yu, Xiaowen Sun, Lin Li*. Nematicidal effects of 2-methyl-aconitate isomerase from the phytopathogen Pseudomonas syringae MB03 on the model nematode Caenorhabditis elegans. Journal of Invertebrate Pathology 2021, 185: 107669.

18. Anum Bashir, Tian Tian, Xun Yu, Cui Meng, Muhammad Ali, Lin Li*. Pyoverdine-mediated killing of Caenorhabditis elegans by Pseudomonas syringae MB03 and the role of iron in its pathogenicity. International Journal of Molecular Sciences 2020, 21: 2198.

19. Zhen Zhang, Zhiyong Ruan, Jin Liu, Chang Liu, Fuming Zhang, Robert J. Linhardt, Lin Li*. Complete degradation of bisphenol A and nonylphenol by a composite of biogenic managanese oxides and Escherichia coli cells with surface-displayed multicopper oxidase CotA. Chemical Engineering Journal 2019, 362: 897-908.

20. Zhen Zhang, Jin Liu, Jin Fan, Zhiyong Wang, Lin Li*. Detection of catechol using an electrochemical biosensor based on engineered Escherichia coli cells that surface-display laccase. Analytica Chimica Acta 2018, 1009: 65-72.

21. Abdul Manan, Zahoor Ahmad Bazai, Jin Fan, Huafu Yu, Lin Li*. The Nif3-family protein YqfO03 from Pseudomonas syringae MB03 has multiple nematicidal activities against Caenorhabditis elegans and Meloidogyne incognita. International Journal of Molecular Sciences 2018, 19, 3915.

22. Zhiyong Wang, Jieping Wang, Jin Liu, Hong Chen, Mingshun Li, Lin Li*. Mechanistic insights into manganese oxidation of a soil-borne Mn(II)-oxidizing Escherichia coli strain by global proteomic and genetic analyses. Scientific Reports 2017, 7: 1352

23. Penggao Hu, Zhongna Zhang, Fei Shen, Xun Yu, Mingshun Li, Hong Ni, Lin Li*. Poly-g-glutamic acid coupled Pseudomonas putida cells surface-displaying metallothioneins: composited copper(II) biosorption and inducible flocculation in aqueous solution. RSC Advances 2017, 7: 18578-18587.

24. Lin Li*, Zhenmin Chen, Ziniu Yu. Mass production, application, and market development of Bacillus thuringiensis biopesticides in China. In: Bacillus thuringiensis and Lysinibacillus sphaericus, Fiuza LM et al (eds.), Springer International Publishing AG 2017. ISBN: 978-3-319-56677-1. pp. 185-212, 2017. DOI: 10.1007/978-3-319-56678-8_12.

25. Xu Qin, Xuemei Xiang, Xiaowen Sun, Hong Ni, Lin Li*. Preparation of nanoscale Bacillus thuringiensis chitinases using silica nanoparticles for nematicide delivery. International Journal of Biological Macromolecules 2016, 82: 13-21.

26. Hua Li, Penggao Hu, Xiuyun Zhao, Ziniu Yu, Lin Li*. Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division. Acta Biochimica et Biophysica Sinica 2016, 48(4): 354-362.

27. Muhammad Ali, Yu Sun, Li Xie, Huafu Yu, Anum Bashir, Lin Li*. The pathogenicity of Pseudomonas syringae MB03 against Caenorhabditis elegans and the transcriptional response of nematicidal genes upon different nutritional conditions. Frontiers in Microbiology 2016, 7: 805.

28. Jin Liu, Luming Tan, Jing Wang, Zhiyong Wang, Hong Ni, Lin Li*. Complete biodegradation of chlorpyrifos by engineered Pseudomonas putida cells expressing surface-immobilized laccases. Chemosphere 2016, 157: 200-207.

29. Zhen Zhang, Zhongming Zhang, Hong Chen, Jin Liu, Chang Liu, Hong Ni, Changsong Zhao, Muhammad Ali, Fan Liu, Lin Li*. Surface Mn(II) oxidation actuated by a multicopper oxidase in a soil bacterium leads to the formation of manganese oxide minerals. Scientific Reports 2015, 5: 10895

30. Hong Ni, Siquan Zeng, Xu Qin, Xiaowen Sun, Shan Zhang, Xiuyun Zhao, Ziniu Yu, Lin Li*. Molecular docking and site-directed mutagenesis of a Bacillus thuringiensis chitinase to improve chitinolytic, synergistic lepidopteran-larvicidal and nematicidal activities. International Journal of Biological Sciences 2015, 11: 304-315.

31. Hong Ni, Zhe Xiong, Ting Ye, Zhen Zhang, Xiaofeng Ma, Lin Li*. Biosorption of copper(II) from aqueous solutions using volcanic rock matrix-immobilized Pseudomonas putida cells with surface-displayed cyanobacterial metallothioneins. Chemical Engineering Journal 2012, 204-205: 264-271.（封面论文）

32. Wei Wang, Zhen Zhang, Hong Ni, Xiaomeng Yang, Qianqian Li, Lin Li*. Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase. Microbial Cell Factories 2012, 11: 75.

33. Qianqian Li, Qi Yan, Jinsi Chen, Yan He, Jing Wang, Hongxing Zhang, Ziniu Yu, Lin Li*. Molecular characterization of an ice nucleation protein variant (InaQ) from Pseudomonas syringae and the analysis of its transmembrane transport activity in Escherichia coli. International Journal of Biological Sciences 2012; 8(8):1097-1108.

34. Xiaohu Shao, Hong Ni, Ting LU, Mengtian Jiang, Hua Li, Xinfeng Huang, Lin Li*. An improved system for the surface immobilisation of proteins on Bacillus thuringiensis vegetative cells and spores through a new spore cortex-lytic enzyme anchor. New Biotechnology 2012, 29(3): 302-310.

35. Lin Li*, Ziniu Yu. Genetically Modified Bacillus thuringiensis Biopesticides. In: Bacillus thuringiensis Biotechnology. Ed. Estibaliz Sansinenea. Springer publishing, 2012. pp. 231-258. ISBN: 978-94-007-3020-5. DOI: 10.1007/978-94-007-3021-2.

36. Mengtian Jiang, Xiaohu Shao, Hong Ni, Ziniu Yu, Lin Li*. In vivo and in vitro surface display of heterologous proteins on Bacillus thuringiensis vegetative cells and spores. Process Biochemistry, 2011, 46: 1861-1866.

37. Qianqian Li, Hong Ni, Shan Meng, Yan He, Ziniu Yu, Lin Li*. Suppressing Erwinia caratovora pathogenicity by projecting N-acyl homoserine lactonase onto the surface of Pseudomonas putida cells. Journal of Microbiology and Biotechnology 2011, 21(12): 1330-1335.

38. Xiaohu Shao, Yinghui Gao, Mengtian Jiang, Lin Li*. Deletion and site-directed mutagenesis of laccase from Shigella dysenteriae results in enhanced enzymatic activity and thermostability. Enzyme and Microbial Technology, 2009, 44: 274-280.

39. Qianqian Li, Ziniu Yu, Xiaohu Shao, Jin He, Lin Li*. Improved phosphate biosorption by bacterial surface display of phosphate-binding protein utilizing ice nucleation protein. FEMS Microbiology Letters, 2009, 299: 44-52.

40. Xiaohu Shao, Mengtian Jiang, Ziniu Yu, Hao Cai, Lin Li*. Surface display of heterologous proteins in Bacillus thuringiensis using a peptidoglycan hydrolase anchor. Microbial Cell Factories 2009, 8(1): 48.

授权发明专利

1.  李林, 谭璐明，王智勇，张震. 一种锰氧化假单胞菌T34及生物氧化锰制备方法和降解环丙沙星的应用. 国家发明专利, 专利号: ZL 201410387871.3.

2. 李林, 汤浪,白志强, 刘瑾. 一种利用壳聚糖为载体制备的生物复合材料及应用.国家发明专利, 专利号: 201510195759.4

3. 李林, 向雪梅. 一种纳米Bt复配杀虫剂及制备方法与应用.国家发明专利, 专利号: ZL 201410189514.6.

4. 李林, 秦旭, 孙孝文, 顾桐, 张震. 一种丁香假单胞菌及其筛选方法和在杀灭线虫中的应用.国家发明专利, 专利号: ZL201410199803.4

5. 李林，白志强，汤浪，张震. 一种壳聚糖珠的制备方法及其制备装置.国家发明专利, 专利号: ZL 201310741611.7

6. 李林, 张润, 丁美娟, 孙孝文, 刘永轩. 防治根结线虫的远东假单胞菌及其制备方法与应用.中华人民共和国国家知识产权局, 国家发明专利, 专利号: ZL 201910752350.6

7.  李林, 李哲, 孙孝文, 曾洁, 李智. 以樟脑为碳源生长的贝莱斯芽孢杆菌及高温堆肥腐熟菌剂和应用. 国家发明专利, 专利号: ZL 202210865553.8