影响因子：3.738

DOI码：10.1016/j.micpath.2022.105389

发表刊物：Microb Pathog

项目来源：National Natural Science Foundation of China (31772791 and 32172914), National Key Research and Deve

关键字：oultry E. coliAntimicrobial resistanceVirulenceβ-LactamaseMultidrug resistanceWhole genome resequencing

摘要：Emergence of multidrug resistance in E. coli and advent of newer strains is becoming serious concern which requires keen observations. This study was designed to find the ciprofloxacin resistant E. coli isolates co-existed with multi-drug resistance along with β-lactamase production from poultry source, and finally the genome sequencing of these strains to explore genetic variations. Study constituted on isolation of n = 225 E. coli from broiler farms of central China which were further subjected to identification of resistance against ciprofloxacin followed by antibiogram of n = 26 antibiotics and identification of β-lactamase production. Whole genome resequencing was performed using Illumina HiSeq 4000 system. PCR results revealed predominant β-lactamase genes i.e.CTX-M, CTX-M-1, CTX-M3, TEM-1 and OXA. Furthermore, the MDR isolates were containing most of the tested virulence genes. The most prevalent virulence genes were pap-C, fim-C, fim-H, iuc-D, irp-2, tra-T, iro-N and iut-A. The single nucleotide polymorphisms (SNPs) loci mentioned in this data give valuable genetic markers to growing high-throughput techniques for fine-determination of genotyping of MDR and virulent isolates. Characterization of SNPs on functional basis shed new bits of knowledge on the evolution, disease transmission and pathogenesis of MDR E. coli isolates. In conclusion, these findings provide evidence that most of poultry E. coli are MDR, β-lactamase producers, and virulent which could be a zoonotic threat to the humans. The whole genome resequencing data provide higher resolution of resistance and virulence characteristics in E. coli which can further be used for the development of prevention and treatment strategies.

合写作者：Yufeng Gu,Qari Muhammad Kaleem,Amjad Islam Aqib,Awais Ihsan,Lingquan Yang,Yulian Wang,Xiuhua Kuang,Mujahid Iqbal

第一作者：Zahid Iqbal,Hafiz Iftikhar Hussain

论文类型：期刊论文

通讯作者：Haihong Hao

论文编号：105389

卷号：163

字数：4000

是否译文：否

发表时间：2022-01-05

收录刊物：SCI

发布期刊链接：https://www.sciencedirect.com/science/article/pii/S088240102200002X?via%3Dihub