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郝海红

博士生导师
硕士生导师
教师姓名:郝海红
教师英文名称:High
教师拼音名称:haohaihong
职称:教授
在职信息:在职
学历:博士研究生毕业
学位:博士
办公地点:华中农业大学国家兽药残留基准实验室118
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毕业院校:华中农业大学
所属院系:动物科学技术学院、动物医学院
所在单位:动物科学技术学院、动物医学院
学科:兽医学其他专业    基础兽医学    
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论文成果
Clinical Breakpoint of Apramycin to Swine Salmonella and Its Effect on Ileum Flora
发布时间:2022-03-03    点击次数:

影响因子:5.924

DOI码:10.3390/ijms23031424

发表刊物:International Journal of Molecular Sciences

关键字:Salmonella, apramycin, wild-type cutoff value, PK/PD cutoff value, clinical cutoff value, clinical breakpoint, 16S rRNA gene sequencing

摘要:The purpose of this study was to establish the clinical breakpoint (CBP) of apramycin (APR) against Salmonella in swine and evaluate its effect on intestinal microbiota. The CBP was established based on three cutoff values of wild-type cutoff value (COWT), pharmacokinetic-pharmadynamic (PK/PD) cutoff value (COPD) and clinical cutoff value (COCL). The effect of the optimized dose regimen based on ex vivo PK/PD study. The evolution of the ileum flora was determined by the 16rRNA gene sequencing and bioinformatics. This study firstly established the COWT, COPD in ileum, and COCL of APR against swine Salmonella, the value of these cutoffs were 32 µg/mL, 32 µg/mL and 8 µg/mL, respectively. According to the guiding principle of the Clinical Laboratory Standards Institute (CLSI), the final CBP in ileum was 32 µg/mL. Our results revealed the main evolution route in the composition of ileum microbiota of diarrheic piglets treated by APR. The change of the abundances of Bacteroidetes and Euryarchaeota was the most obvious during the evolution process. Methanobrevibacter, Prevotella, S24-7 and Ruminococcaceae were obtained as the highest abundance genus. The abundance of Methanobrevibacter increased significantly when APR treatment carried and decreased in cure and withdrawal period groups. The abundance of Prevotella in the tested groups was significantly lower than that in the healthy group. A decreased of abundance in S24-7 was observed after Salmonella infection and increased slightly after cure. Ruminococcaceae increased significantly after Salmonella infection and decreased significantly after APR treatment. In addition, the genera of Methanobrevibacter and Prevotella were defined as the key node. Valine, leucine and isoleucine biosynthesis, D-Glutamine and D-glutamate metabolism, D-Alanine metabolism, Peptidoglycan and amino acids biosynthesis were the top five Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in the ileum microbiota of piglets during the Salmonella infection and APR treatment process. Our study extended the understanding of dynamic shift of gut microbes during diarrheic piglets treated by APR.

合写作者:Jinli Guo,Lingli Huang,Guyue Cheng

第一作者:Yufeng Gu,Xinyu Dai

论文类型:期刊论文

通讯作者:Dapeng Peng,Haihong Hao

卷号:23

期号:3

页面范围:142

是否译文:

发表时间:2022-02-01

收录刊物:SCI

发布期刊链接:https://doi.org/10.3390/ijms23031424