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陈小林

Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
Name (Simplified Chinese):陈小林
Name (Pinyin):chenxiaolin
E-Mail:
School/Department:植物科学技术学院
Education Level:With Certificate of Graduation for Doctorate Study
Business Address:分子楼111
Degree:Doctoral degree
Professional Title:Professor
Status:Employed
Alma Mater:中国农业大学
Teacher College:College of Plant Sciences & Technology
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陈小林:男,1981年8月出生,教授,博士生导师,湖北荆门人,农业微生物学国家重点实验室固定研究人员,华中农业大学植物科学技术学院教师。在植物病理教研室从事教学和科研工作,主讲本科生《分子植物病理学》和研究生《植物病原真菌致病分子机制》。长期从事稻瘟病研究工作,中国植物保护学会青年委员会委员。以通讯作者或第一作者在Ann Rev Phytopathol,Plant Cell,New Phytol,Plant Biotechnol J,PLOS Pathog,PLOS Genet,ACS Appl Mater Interfaces,Environ Microbiol,Mol Plant Pathol,Front Microbiol,Front Plant Sci等期刊发表论文29篇,先后主持国家自然科学基金4项。

学习工作经历:

2000.9-2004.6:华中农业大学植物保护系,本科

2004.9-2011.6:中国农业大学植物病理学专业,硕博连读

2011.7-2014.10:中国农业大学作物遗传育种专业,博士后

2014.10-2015.3:北京市农林科学院,科研助理

2015.3-2021.11:华中农业大学,副教授

2019.10-2020.8:英国The Sainsbury Laboratory/John Inners Centre,访问学者

2021.12-至今:华中农业大学,教授

研究内容

以稻瘟菌-水稻作为模式系统,进行真菌致病分子机制,水稻抗病分子机制,以及病原真菌-植物互作分子机制研究。结合正向遗传学和反向遗传学策略,以解析致病过程分子调控机制为目的,综合运用功能基因组学,分子遗传学,分子细胞生物学,修饰蛋白组学,表观遗传修饰组学,多组学技术等手段进行研究。研究成果可为其它非模式病原真菌和非模式寄主植物提供借鉴,为开发新型杀菌剂提供候选靶标,为发现新的真菌病害防控途径,乃至培育抗病品种提供理论依据和应用基础。

当前主要研究方向:

1)植物病原真菌翻译后修饰调控机制

2)植物病原真菌表观遗传学调控机制

3)植物病原真菌分子细胞生物学调控机制

4)植物病原真菌免疫逃避机制

5)基于多组学技术的植物抗病机制

6)杀菌活性物质和小分子化合物筛选/绿色杀菌剂开发

 

科研项目

1.         华中农业大学校自主科技创新基金(优秀人才培育项目),稻瘟菌表观遗传和蛋白修饰调控机制,课题编号:2021ZKPY007,项目年限:2021/5-2024/4(主持)

2.         科技部,国家自然科学基金面上项目,O-GlcNAc糖基化修饰调控稻瘟菌致病过程的分子机制研究. 课题编号:32072365, 项目年限:2021/1-2024/12(主持)

3.         科技部,国家自然科学基金面上项目,SUMO化修饰调控稻瘟菌MAPK信号途径的分子机制研究,课题编号:31871909,项目年限:2019/1-2022/12(主持)

4.         科技部,国家自然科学基金青年项目,GPI锚定修饰在稻瘟菌致病过程中的作用和机理研究,课题编号:31601585,项目年限:2017/1-2019/12(主持)

5.         科技部,国家自然科学基金面上项目,稻瘟菌酸性pH信号途径的鉴定及其在致病过程中的作用研究,课题编号:31571952,项目年限:2016/1-2019/12(主持)

6.         华中农业大学校自主科技创新基金(人才培育项目),翻译后修饰在稻瘟菌致病过程中的作用机理研究,课题编号:2662015PY085,项目年限:2016/1-2019/12(主持)

7.         华中农业大学校自主科技创新基金(新进教师科研启动专项),稻瘟菌两种糖基化修饰系统的比较研究,课题编号:0900206306,项目年限:2015/1-2017/12(主持)

 

教学研究与教学改革

1.       2019年院级教改项目:新形势下本科“分子植物病理学”课程改革与创新(主持)

2.       教改论文:陈小林*,董五辈,郑露,杨龙,现代农业背景下植物病理专业“分子植物病理学”课程改革与创新. 教育教学论坛. 2020. 39: 193-194.

 

发明专利及获得奖励

主要奖励:

1.           2017年华中农业大学教学质量优秀二等奖

2.           2015年植物科学技术学院教学质量优秀奖

3.           华中农业大学学士学位论文创新奖指导教师(2019)

4.           华中农业大学优秀本科毕业论文指导教师(2020)

5.           华中农业大学本科生SRF项目指导教师(2017,2018,2019,2021)

6.           华中农业大学本科生省创项目指导教师(2021)

7.           华中农业大学本科生科技创新专项指导教师(2017)

8.           指导研究生获得华中农业大学优秀硕士毕业论文(2018,2019)

9.           指导研究生获得国家奖学金(2019,2020,2021)

10.       指导研究生获得华中农业大学科研创新奖(2018)

11.       指导研究生获得农业微生物学国家重点实验室年度优秀研究生(2021)

 

学术论文与著作(*表示通讯作者,#表示共同作者):

第一或通讯作者文章

1.       Liu C.Y., Liu T., Lv ZW, Qin M.Y., Qu Z.G., Zhang Z.W., Li F.Y., Chen D., Zhang X.R., Chen X.L.*, Shen M.* 2022. A calcineurin regulator MoRCN1 is important for asexual development, stress response and plant infection of Magnaporthe oryzae. Frontiers in Plant Science, Accepted.

2.       Ren, Z.#, Tang, B.#, Xing, J., Liu, C., Cai, X., Hendy, A., Kamran, M., Liu, H., Zheng, L., Huang, J., Chen, X.L.* 2022. MTA1-mediated RNA m6 A modification regulates autophagy and is required for infection of the rice blast fungus. New Phytologist, 10.1111/nph.18117. Advance online publication.

3.       Cai, X., Xiang, S., He, W., Tang, M., Zhang, S., Chen, D., Zhang, X., Liu, C., Li, G., Xing, J., Li, Y.*, Chen, X.L.*, & Nie, Y.* 2022. Deubiquitinase Ubp3 regulates ribophagy and deubiquitinates Smo1 for appressorium-mediated infection by Magnaporthe oryzae. Molecular Plant Pathology, 10.1111/mpp.13196. Advance online publication.

4.       Zhao, J., Sun, P., Sun, Q., Li, R., Qin, Z., Sha, G., Zhou, Y., Bi, R., Zhang, H., Zheng, L., Chen, X.L., Yang, L., Li, Q., Li, G.* 2022. The MoPah1 phosphatidate phosphatase is involved in lipid metabolism, development, and pathogenesis in Magnaporthe oryzae. Molecular Plant Pathology, 23(5), 720–732.

5.       Mei, J., Ning, N., Wu, H., Chen, X.L., Li, Z.*, Liu, W.* 2022. Glycosylphosphatidylinositol Anchor Biosynthesis Pathway-Related Protein GPI7 Is Required for the Vegetative Growth and Pathogenicity of Colletotrichum graminicola. International Journal of Molecular Sciences, 23(6), 2985.

6.       Chen, D.#, Hu, H.#, He, W., Zhang, S., Tang, M., Xiang, S., Liu, C., Cai, X., Hendy, A., Kamran, M, Liu, H., Zheng, L., Huang, J., Chen, X.L.*, Xing J.* 2021. Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae. Molecular Plant Pathology, doi: 10.1111/mpp.13149.

7.       Tang, B., Liu, C., Li, Z., Zhang, X., Zhou, S., Wang, G.L., Chen, X.L.*, Liu, W.D.* 2021. Multilayer regulatory landscape during pattern-triggered immunity in rice. Plant Biotechnology Journal, 19(12):2629-2645.

8.       Chen, X., Pei, Z., Liu, H., Huang, J., Chen, X.L., Luo, C., Hsiang, T., Zheng, L.* 2021. Host-induced gene silencing of fungal-specific genes of Ustilaginoidea virens confers effective resistance to rice false smut. Plant Biotechnology Journal, doi: 10.1111/pbi.13756.

9.       Chen, X., Pei, Z., Peng, L., Qin, Q., Duan, Y., Liu, H., Chen, X.L., Zheng, L., Luo C., Huang, J.* 2021. Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in Ustilaginoidea virens. Journal of Fungi, doi:10.3390/jof7110947.

10.     Liu W.D.*, Triplett L.*, Chen X.L.* 2021. Emerging roles of posttranslational modifications in plant-pathogenic fungi and bacteria. Annual Review of Phytopathology, 2021(59). 59: 99-124.

11.     Liu C.Y., Talbot N.J.*, Chen X.L.* 2021. Protein glycosylation during infection by plant pathogenic fungi. New Phytologist. 230(4): 1329-1335.

12.     Peng, Z., Li, L., Wu, S., Chen, X.L., Shi, Y., He, Q., Shu, F., Zhang, W., Sun, P., Deng, H., Xing, J.* 2021. Frequencies and variations of Magnaporthe oryzae avirulence genes in Hunan Province, China. Plant disease, doi:10.1094/PDIS-01-21-0008-RE.

13.     Chen, X., Li, P., Liu, H., Chen, X.L., Huang, J., Luo, C., Li, G., Hsiang, T., Collinge, D. B., Zheng, L. (2021). A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens. Virulence, 12(1), 1563–1579.

14.     Chen, X., Pei, Z., Li, P., Li, X., Duan, Y., Liu, H., Chen, X.L., Zheng, L., Luo, C., & Huang, J. (2021). Quantitative proteomics analysis reveals the function of the putative ester cyclase UvEC1 in the pathogenicity of the rice false smut fungus Ustilaginoidea virens. International Journal of Molecular Sciences, 22(8), 4069.

15.     Chen X.Y., Liu H., Chen X.L., Huang J.B., Hsiang T., Zheng L. 2021. ATAC-seq data for genome-wide profiling of transcription factor binding sites in the rice false smut fungus Ustilaginoidea virens. Molecular Plant-Microbe Interactions MPMI01210006A.

16.     Zhang X.R.#, Zhang Z.H.#, Chen X.L.* 2021. The redox proteome of thiol proteins in the rice blast fungus Magnaporthe oryzae. Frontiers in Microbiology. 12:648894.

17.     Zhang X.R., Chen X.L.* 2021. The emerging roles of ubiquitin-like protein Urm1 in eukaryotes. Cellular Signalling. 81 (2021) 109946.

18.     Li X.H., Zhao H.J., Chen X.L.* 2021. Screening of marine bioactive antimicrobial compounds for plant pathogens. Marine Drugs. 2021, 19, 69.

19.     Chen, X., Xu, Q., Duan, Y., Liu, H., Chen, X.L., Huang, J., Luo, C., Zhou, D. X., Zheng, L.* 2021. Ustilaginoidea virens modulates lysine 2-hydroxyisobutyrylation in rice flowers during infection. Journal of Integrative Plant Biology, 63(10), 1801–1814.

20.     Chen X.Y., Li X.B., Li P.P., Chen X.L., Liu H., Huang J.B., Luo C.X., Hsiang T., Zheng L. 2021. Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement. Journal of Integrative Plant Biology, 63(2):409-425.

21.     Chen X.L., Liu C.Y., Tang B.Z., Ren Z.Y., Wang G.L. and Liu W.D.* 2020. Quantitative proteomics analysis reveals important roles of N-glycosylation on ER quality control system for development and pathogenesis in Magnaporthe oryzae. PLOS Pathogens, 16(2): e1008355.

22.     Cai X., Yan J., Liu C., Xing J., Ren Z., Hendy A., Zheng L., Huang J., Chen X.L.* 2020. Perilipin LDP1 coordinates lipid droplets formation and utilization for appressorium-mediated infection in Magnaporthe oryzae. Environmental Microbiology, 22(7):2843-2857.

23.     Liu C.Y., Xing J.J., Cai X., Hendy A., He W.H., Yang J., Huang J.B., Peng Y.L., Ryder L., Chen X.L.* 2020. GPI7-mediated glycosylphosphatidylinositol (GPI) anchoring regulates appressorial penetration and immune evasion during infection of Magnaporthe oryzae. Environmental Microbiology, 22(7):2581-2595. DOI: 10.1111/1462-2920.14941.

24.     Cai X.#, Wang Z.#, Hou Y.X., Liu C., Hendy A., Xing J., Chen X.L.* 2020. Systematic characterization of the ubiquitin-specific proteases in Magnaporthe oryzae. Phytopathology Research, 8 (2020).

25.     Li S.J.#, Song Z.Y.#, Liu C.Y., Chen X.L.*, Han H.Y.* 2019. Biomimetic mineralization-based CRISPR/Cas9 ribonucleoprotein nanoparticles for gene editing. ACS Applied Materials & Interfaces. 11(51):47762-47770.

26.     Wang L.Y.#, Cai X.#, Xing J.J., Liu C.Y., Hendy A., Chen X.L.* 2019. URM1-mediated ubiquitin-like modification is required for oxidative stress adaptation during infection of the rice blast fungus. Frontiers in Microbiology, 10: 2039.

27.     Chen F.F., Ma R.J., Chen X.L.* 2019. Advances of metabolomics in fungal pathogen–plant interactions. Metabolites, 2019(9): 169.

28.     Hendy A., Xing J.J., Chen X.Y., Chen X.L.* 2019. The farnesyltransferase β-subunit RAM1 regulates localization of RAS proteins and appressorium-mediated infection in Magnaporthe oryzae. Molecular Plant Pathology, 20(9): 1264-1278.

29.     Liu C.Y.#, Li Z.G.#, Xing J.J., Yang J., Wang Z., Zhang H., Chen D., Peng Y.L., Chen X.L.* 2018. Global analysis of sumoylation function reveals novel insights into development and appressorium-mediated infection of the rice blast fungus. New Phytologist. 219: 1031-1047.

30.     Chen X.L., Xie X., Liu C.Y., Zeng L.R., Zhou X.P., Luo F., Wang G.L.*, Liu W.D.* 2018. Proteomic analysis of ubiquitinated proteins in rice (Oryza sativa) after treatment with PAMP elicitors. Frontiers in Plant Science, (2018)9: 707.

31.     Wang Z., Zhang H., Liu C.Y., Xing J.J., Chen X.L.* 2018. A deubiquitinating enzyme Ubp14 is required for development, stress response, nutrient utilization, and pathogenesis of Magnaporthe oryzae. Frontiers in Microbiology, (2018)9: 769.

32.     Yuan J.L., Wang Z., Xing J.J., Yang Q.Y., Chen X.L.* 2018. Genome-wide Identification and characterization of circular RNAs in the rice blast fungus Magnaporthe oryzae. Scientific Reports, (2018)8: 6757.

33.     Chen X.L.* 2018. Infection process observation of Magnaporthe oryzae on barley leaves. Bio-protocol, Vol 8, Iss 08. doi: 10.21769/BioProtoc.2833.

34.     Li L.W.#, Chen X.L.#, Zhang S.P., Yang J., Chen D., Liu M.X., Zhang H.F., Zheng X.B., Wang P., Peng Y.L.*, Zhang Z.G.* 2017. MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae. PLoS Genetics, 13(5): e1006814.

35.     Chen X.L.#*, Shen M.#, Yang J., Xing Y.F., Chen D., Li Z.G., Zhao W.S., Zhang Y. 2017. Peroxisomal fission is induced during appressorium formation and is required for full virulence of the rice blast fungus. Molecular Plant Pathology, 18(2):222-237.

36.     Zhou W., Shi W., Xu X.W., Li Z.G., Yin C.F., Peng J.B., Chen X.L., Zhao W.S., Zhang Y., Yang J.*, Peng Y.L. 2017. Glutamate synthase is required for conidiation, full virulence, and autophagy in the rice blast fungus, Molecular Plant Pathology, 18(2): 222-237.

37.     Yang J., Tsiang T., Bhadauria V., Chen X.L., Li G.T. 2017. Plant Fungal Pathogenesis. Editorial. BioMed Res Int, 2017:9724283.

38.     Chen X.L.*, Wang Z., Liu C.Y. 2016. Roles of peroxisomes in the rice blast fungus. BioMed Res Int, 2016:9343417.

39.     Wang Y., He D., Chu Y., Zuo Y.S., Xu X.W., Chen X.L., Zhao W.S., Zhang Y., Yang J.*, Peng Y.L. 2016. MoCps1 is important for conidiation, conidial morphology and virulence in Magnaporthe oryzae. Current Genetics, 62(4): 861-871.

40.     Chen X.L., Shi T., Yang J., Chen D., Xu X.W., Xu J.R., Talbot N.J., Peng Y.L* 2014. N-glycosylation of effector proteins by an alpha-1,3-mannosyltransferase is required to evade host innate immunity by the rice blast fungus. The Plant Cell, 26: 1360-1376.

41.     Li C., Yang J., Zhou W., Chen X.L., Huang J.G., Cheng Z.H., Zhao W.S., Zhang Y. Peng Y.L.* 2014. A spindle pole antigen gene MoSPA2 is important for polar cell growth of vegetative hyphae and conidia, but is dispensable for pathogenicity in Magnaporthe oryzae. Current Genetics, 60(4): 255-263.

42.     Zuo Y.S., Yang J., Wang D.W., He D., Chu Y., Chen X.L., Zhou W., Hsiang T., Peng Y.L.* 2014. MoTlg2, a t-SNARE component is important for formation of the Spitzenkörper and polar deposition of chitin in Magnaporthe oryzae. Physiological and Molecular Plant Pathology, 87:9-18.

43.     Du Y.X., Shi Y., Yang J., Chen X.L., Xue M.F., Zhou W.S., Peng Y.L.* 2013. A serine/threonine-protein phosphatase PP2A catalytic subunit is essential for asexual development and plant infection in Magnaporthe oryzae. Current Genetics, 59(1-2): 33-41.

44.     Yang J., Kong L.A., Chen X.L., Wang D.W., Qi L.L., Zhao W.S., Zhang Y, Liu X.Z., Peng Y.L.* 2012. A carnitine-acylcarnitine carrier protein, MoCrc1, is essential for pathogenicity in Magnaporthe oryzae. Current Genetics, 58(3):139-148.

45.     Chen X.L., Yang J., and Peng Y.L.* 2011. Large-scale insertional mutagenesis in Magnaporthe oryzae by Agrobacterium tumefaciens-mediated transformation. Methods in Molecular Biology, 722: 213-224.


Educational Experience

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Work Experience

[1] 2015.3-Now
华中农业大学植物科学技术学院
教学科研 

[2] 2011.7-2014.10
中国农业大学
博士后 

Research Focus

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