张金智,中国民主同盟盟员,博士、教授、博士生导师,华中农业大学果树系研究生毕业,获博士学位,留校任教。2013年-2014年赴美国加利福尼亚大学圣地亚哥分校赵云德实验室访问交流。一直从事果树发育分子生物学研究。博士学位论文荣获全国百篇优秀博士论文提名。目前以第一作者或通讯作者在SCI源刊杂志上发表40余篇,获批国家专利六项。主持国家自然科学基金、国际科学基金、国家重大研发计划子课题,教育部新教师基金和华中农业大学自主基金等项目。主持和参与多项省级和校级教改项目,指导大学生参加学科竞赛荣获得国家一等奖一项、二等奖一项,湖北特等奖两项。指导大学生科研论文成果奖一等奖一项,二等奖一项,湖北优秀学位论文两篇。
主要从事柑橘发育分子生物学机理与遗传改良研究。
研究重点:(1)柑橘童期与花器官发育分子机理解析,缩短育种周期、提早果树结果。(2)橘株型发育的机制及调控:以柑橘新梢自剪为切入点,解析果树顶端组织和茎间分生组织发生、发育调控的机制,开发调控果树株型的技术措施,为果树优质丰产和省力化栽培奠定基础。(3)柑橘成花调控的机制及其应用:生产上常采用水分胁迫和激素等措施调控柑橘花期,进而达到调控果实成熟期的目的,所以实验室致力于解析其机制与应用。
(一)获得奖励
1. 2013年指导本科生的科研成果荣获湖北省大学生优秀科研成果一等奖;
2. 2013年指导本科生的课外作品荣获第九届湖北省“挑战杯”大学生课外学术科技作品竞赛特等奖;
3. 2013年指导本科生的课外作品荣获第十三届“挑战杯”交通银行全国大学生课外学术科技作品竞赛二等奖;
4. 2015年指导本科生的科研成果荣获湖北省大学生优秀科研成果二等奖;
5. 2015年指导本科生的课外作品荣获第十届湖北省“挑战杯”大学生课外学术科技作品竞赛特等奖;
6. 2016年指导本科生的学术论文荣获第三届全国植物生产类大学生实践创新一等奖;
7. 2016年荣获华中农业大学科技创新优秀指导教师;
8. 2014年荣获湖北省优秀学士学位论文指导教师;
9. 2015年荣获湖北省优秀学士学位论文指导教师;
10. 2017年荣获华中农业大学校级优秀硕士学位论文指导奖;
11. 博士论文《利用抑制性差减杂交研究早实枳成花转变的分子机理》荣获2012年全国百篇优秀博士论文提名奖;
12.《PtFLC homolog from trifoliate orange (Poncirus trifoliata) is regulated by alternative splicing and experiences seasonal fluctuation in expression level》于2010年荣获湖北省第十三届自然科学优秀学术论文二等奖。
(二)授权专利
1. 张金智;徐晓玲;艾小艳;胡春根。一个水杨酸诱导的红肉脐橙启动子GSTU19P的分离及应用,专利号:201110028718.8;
2. 张金智;胡春根。柑橘早花基因PtELF5的分离克隆及应用, 专利号:201110028785.X;
3. 张金智;侯晓进;胡春根。甜橙根特异性启动子CsBFTP的分离及应用, 专利号:201410665913.;。
4. 张金智;胡春根;侯小进。甜橙CsMYB120基因及其应用,专利号:2019111658756;
5. 张金智;胡春根;侯小进。克里曼丁花特异性表达启动子CcPIpro及其应用,专利号:201911167030.0;
6. 张金智;胡春根;叶丽霞;朱胜龙;张金霞;曾仁芳。柑橘bZIP转录因子在缩短植物童期中的应用,专利号:CN202110139779.5。
(三)发表文章
1. L-X Ye Y-M Wu, J-X Zhang, J-X Zhang, H Zhou, R-F Zeng, W-X Zheng, M-Q Qiu, J-J Zhou, Z-Z Xie, C-G Hu, J-Z Zhang*: A bZIP transcription factor (CiFD) regulates drought and low temperature induced flowering by alternative splicing in citrus, Journal of Integrative Plant Biology (3): 674-691.
2. Chu L-L, Yan Z, Sheng X-X, Liu H-Q, Wang Q-Y, Zeng R-F, Hu C-G, Zhang J-Z*: Citrus ACC synthase CiACS4 regulates plant height by inhibiting gibberellin biosynthesis. Plant Physiology 2023:kiad159.
4. Zhou H, Zeng R-F, Liu T-J, Ai X-Y, Ren M-K, Zhou J-J, Hu C-G*, Zhang J-Z*: Drought and low temperature-induced NF-YA1 activates FT expression to promote citrus flowering. Plant, Cell & Environment 2022, 45(12):3505-3522.
5. Zeng R-F, Fu L-M, Deng L, Liu M-F, Gan Z-M, Zhou H, Hu S-F, C-G Hu*, Zhang J-Z*: CiKN1 and CiKN6 are involved in leaf development in citrus by regulating CimiR164. The Plant Journal 2022, 110(3):828-848.
6. Xu Y-Y, Zeng R-F, Zhou H, Qiu M-Q, Gan Z-M, Yang Y-L, Hu S-F, Zhou J-J, Hu C-G*, Zhang J-Z*: Citrus FRIGIDA cooperates with its interaction partner dehydrin to regulate drought tolerance. The Plant Journal 2022, 111(1):164-182.
7. Wu Y-M, Ma Y-J, Wang M, Zhou H, Gan Z-M, Zeng R-F, Ye L-X, Zhou J-J, Zhang J-Z*, Hu C-G*: Mobility of FLOWERING LOCUS T protein as a systemic signal in trifoliate orange and its low accumulation in grafted juvenile scions. Horticulture Research 2022, 9:uhac056.
8. Khan FS, Gan Z-M, Li E-Q, Ren M-K, Hu C-G*, Zhang J-Z*: Transcriptomic and physiological analysis reveals interplay between salicylic acid and drought stress in citrus tree floral initiation. Planta 2022, 255(1):24.
9. Hou X-J, Ye L-X, Ai X-Y, Hu C-G, Cheng Z-P*, Zhang J-Z*: Functional analysis of a PISTILLATA-like gene CcMADS20 involved in floral organs specification in citrus. Plant Science 2022, 319:111263.
11.Zeng R-F, Zhou H, Fu L-M, Yan Z, Ye L-X, Hu S-F, Gan Z-M, Ai X-Y, Hu C-G*, Zhang J-Z*: Two citrus KNAT-like genes, CsKN1 and CsKN2, are involved in the regulation of spring shoot development in sweet orange. Journal of Experimental Botany 2021, 72(20):7002-7019.
12.Ye L-X, Zhang J-X, Hou X-J, Qiu M-Q, Wang W-F, Zhang J-X, Hu C-G, Zhang J-Z*: A MADS-box gene CiMADS43 is involved in citrus flowering and leaf development through interaction with CiAGL9. International Journal of Molecular Sciences 2021, 22(10):5205.
13. Xu Y-Y, Liu S-R, Gan Z-M, Zeng R-F, Zhang J-Z*, Hu C-G*: High-density genetic map construction and identification of QTLs controlling leaf abscission trait in Poncirus trifoliata. International Journal of Molecular Sciences 2021, 22(11):5723.
14. Shafique Khan F, Zeng R-F, Gan Z-M, Zhang J-Z*, Hu C-G*: Genome-wide identification and expression profiling of the WOX gene family in citrus sinensis and functional analysis of a CsWUS member. International Journal of Molecular Sciences 2021, 22(9):4919.
15. Ye L-X, Gan Z-M, Wang W-F, Ai X-Y, Xie Z-Z, Hu C-G, Zhang J-Z*: Comparative analysis of the transcriptome, methylome, and metabolome during pollen abortion of a seedless citrus mutant. Plant Molecular Biology 2020, 104(1-2):151-171.
17. Ma Y-J, Li P-T, Sun L-M, Zhou H, Zeng R-F, Ai X-Y, Zhang J-Z*, Hu C-G*: HD-ZIP I transcription factor (PtHB13) negatively regulates citrus flowering through binding to FLOWERING LOCUS C promoter. Plants 2020, 9(1).
18. Zeng R-F, Zhou J-J, Liu S-R, Gan Z-M, Zhang J-Z*, Hu C-G: Genome-wide identification and characterization of SQUAMOSA-Promoter-Binding Protein (SBP) genes involved in the flowering development of Citrus Clementina. Biomolecules 2019, 9(2):66.
19. Zeng R-F, Zhou J-J, Hu C-G*, Zhang J-Z*: Transcriptome-wide identification and functional prediction of novel and flowering-related circular RNAs from trifoliate orange (Poncirus trifoliata L. Raf.). Planta 2018, 247(5):1191-1202.
20. Luo J, Zhou J-J*, Zhang J-Z*: Aux/IAA gene family in plants: molecular structure, regulation, and function. International Journal of Molecular Sciences 2018, 19(1):259.
21. Liu T-J, Zhou J-J, Chen F-Y, Gan Z-M, Li Y-P, Zhang J-Z*, Hu C-G*: Identification of the genetic variation and gene exchange between Citrus trifoliata and Citrus clementina. Biomolecules 2018, 8(4):182.
23. Wang CY, Liu SR, Zhang XY, Ma YJ, Hu CG, Zhang JZ*: Genome-wide screening and characterization of long non-coding RNAs involved in flowering development of trifoliate orange (Poncirus trifoliata L. Raf.). Scientific Reports 2017, 7:43226.
24. Liu S-R, Zhou J-J, Hu C-G, Wei C-L*, Zhang J-Z*: MicroRNA-mediated gene silencing in plant defense and viral counter-defense. Frontiers in Microbiology 2017, 8:1801.
25. Li J-X, Hou X-J, Zhu J, Zhou J-J*, Huang H-B, Yue J-Q, Gao J-Y, Du Y-X, Hu C-X, Hu C-G, Zhang J-Z*: Identification of genes associated with lemon floral transition and flower development during floral inductive water deficits: a hypothetical model. Frontiers in Plant Science 2017, 8:1013.
26. Zhang JZ, Liu SR, Hu CG: Identifying the genome-wide genetic variation between precocious trifoliate orange and its wild type and developing new markers for genetics research. DNA Research 2016, 23(4):403-414.
27. Sun L-M, Zhang J-Z*, Hu C-G*: Characterization and expression analysis of PtAGL24, a SHORT VEGETATIVE PHASE/AGAMOUS-LIKE 24 (SVP/AGL24)-type MADS-box gene from trifoliate orange (Poncirus trifoIlata L. Raf.). Frontiers in Plant Science 2016, 10(7):823.
28. Liu SR, Hu CG, Zhang JZ*: Regulatory effects of cotranscriptional RNA structure formation and transitions. Wiley Interdisciplinary Reviews: RNA 2016, 7(5):562-574.
29. Li S-B, Xie Z-Z, Hu C-G, Zhang J-Z*: A review of auxin response factors (ARFs) in plants. Frontiers in Plant Science 2016, 7:47.
30. Ai X-Y, Zhang J-Z, Liu T-J, Hu C-G*: PtFCA from precocious trifoliate orange is regulated by alternative splicing and affects flowering time and root development in transgenic Arabidopsis. Tree Genetics & Genomes 2016, 12(5):85.
31. Li S-B, OuYang W-Z, Hou X-J, Xie L-L, Hu C-G, Zhang J-Z*: Genome-wide identification, isolation and expression analysis of auxin response factor (ARF) gene family in sweet orange (Citrus sinensis). Frontiers in Plant Science 2015, 6:119.
32. Zhang J-Z, Zhao K, Ai XY, Hu CG: Involvements of PCD and changes in gene expression profile during self-pruning of spring shoots in sweet orange (Citrus sinensis). BMC Genomics 2014, 15:892.
33. Zhang JZ, Mei L, Liu R, Khan MR, Hu CG: Possible involvement of locus-specific methylation on expression regulation of LEAFY homologous gene (CiLFY) during precocious trifoliate orange phase change process. PLoS ONE 2014, 9(2):e88558.
34. Sun LM, Zhang J-Z*, Mei L, Hu CG: Molecular cloning, promoter analysis and functional characterization of APETALA 1-like gene from precocious trifoliate orange (Poncirus trifoliata L. Raf.). Scientia Horticulturae 2014, 178 (23):95-105.
35. Khan MRG, Ai X-Y, Zhang J-Z*: Genetic regulation of flowering time in annual and perennial plants. Wiley Interdisciplinary Reviews: RNA 2014, 5(3):347-359.
36. Hou XJ, Li SB, Liu SR, Hu CG, Zhang JZ*: Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange. PLoS ONE 2014, 9(11):e112375.
37. Liu SR, Li WY, Long D, Hu CG*, Zhang JZ*: Development and characterization of genomic and expressed SSRs in citrus by genome-wide analysis. PLoS ONE 2013, 8(10):e75149.
38. Hou X-J, Liu S-R, Khan M, Hu C-G, Zhang J-Z*: Genome-wide identification, classification, expression profiling, and SSR marker development of the MADS-Box gene family in citrus. Plant Molecular Biology Reporter 2013, 32(1):28-41.
39. Zhang J-Z, Ai X-Y, Guo W-W, Peng S-A, Deng X-X, Hu C-G: Identification of miRNAs and their target genes using deep sequencing and degradome analysis in trifoliate orange [Poncirus trifoliate (L.) Raf]. Molecular Biotechnology 2012, 51(1):44-57.
40. Sun LM, Ai XY, Li WY, Guo WW, Deng XX, Hu CG*, Zhang JZ*: Identification and comparative profiling of miRNAs in an early flowering mutant of trifoliate orange and its wild type by genome-wide deep sequencing. PLoS ONE 2012, 7(8):e43760.
41. Ai X-Y, Lin G, Sun L-M, Hu C-G, Guo W-W, Deng X-X, Zhang J-Z*: A global view of gene activity at the flowering transition phase in precocious trifoliate orange and its wild-type [Poncirus trifoliata (L.) Raf.] by transcriptome and proteome analysis. Gene 2012, 510(1):47-58.
42. Zhang J-Z, Ai X-Y, Sun L-M, Zhang D-L, Guo W-W, Deng X-X, Hu C-G: Molecular cloning and functional characterization of genes associated with flowering in citrus using an early-flowering trifoliate orange (Poncirus trifoliata L. Raf.) mutant. Plant Molecular Biology 2011, 76(1-2):187-204.
43. Zhang J-Z, Ai X-Y, Sun L-M, Zhang D-L, Guo W-W, Deng X-X, Hu C-G: Transcriptome profile analysis of flowering molecular processes of early flowering trifoliate orange mutant and the wild-type [Poncirus trifoliata (L.) Raf.] by massively parallel signature sequencing. BMC Genomics 2011, 12:63.
44. Li Z-M, Zhang J-Z(Co author), Mei L, Deng X-X, Hu C-G, Yao J-L: PtSVP, an SVP homolog from trifoliate orange (Poncirus trifoliata L. Raf.), shows seasonal periodicity of meristem determination and affects flower development in transgenic Arabidopsis and tobacco plants. Plant Molecular Biology 2010, 74(1-2):129-142.
45. Zhang J-Z, Li Z-M, Yao J-L, Hu C-G: Identification of flowering-related genes between early flowering trifoliate orange mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.) by suppression subtraction hybridization (SSH) and macroarray. Gene 2009, 430(1-2):95-104.
46. Zhang J-Z, Li Z-M, Mei L, Yao J-L, Hu C-G: PtFLC homolog from trifoliate orange (Poncirus trifoliata) is regulated by alternative splicing and experiences seasonal fluctuation in expression level. Planta 2009, 229(4):847-859.
47. Zhang J-Z, Li Z-M, Liu L, Mei L, Yao J-L, Hu C-G: Identification of early-flower-related ESTs in an early-flowering mutant of trifoliate orange (Poncirus trifoliata) by suppression subtractive hybridization and macroarray analysis. Tree Physiology 2008, 28:1449–1457.