影响因子：5.754

发表刊物：Frontiers in Plant Science

摘要：Sap-sucking insects cause severe damage to cotton production. Long non-coding RNAs (lncRNAs) play vital regulatory roles in various development processes and stress response, however, the function of lncRNAs during sap-sucking insect infection in cotton is largely unknown. In this study, the transcriptome profiles between resistant (HR) and susceptible (ZS) cotton cultivars under whitefly infestation at different time points (0, 4, 12, 24, and 48 h) were compared. A total of 6,651 lncRNAs transcript and 606 differentially expressed lncRNAs were identified from the RNA-seq data. A co-expression network indicated that lncA07 and lncD09 were potential hub genes that play a regulatory role in cotton defense against aphid infestation. Furthermore, CRISPR/Cas9 knock-out mutant of lncD09 and lncA07 showed a decrease of jasmonic acid (JA) content, which potentially lead to increased susceptibility toward insect infestation. Differentially expressed genes between wild type and lncRNA knock-out plants are enriched in modulating development and resistance to stimulus. Additionally, some candidate genes such as Ghir_A01G022270, Ghir_D04G014430, and Ghir_A01G022270 are involved in the regulation of the JA-mediated signaling pathway. This result provides a novel insight of the lncRNA role in the cotton defense system against pests.

第一作者：Zhang J, Li J, Saeed S, Batchelor WD, Alariqi M, Meng Q, Zhu F, Zou J, Xu Z, Si H, Wang Q, Zhang X, Zhu H, Jin S, Yuan D

论文类型：期刊论文

论文编号：fpls.2022.784511

卷号：13

是否译文：否

发表时间：2022-02-23

收录刊物：SCI

发布期刊链接：https://www.frontiersin.org/articles/10.3389/fpls.2022.784511