Published research papers:

1.Sun L, Zhao C, Fu Z, Fu Y, Su Z, Li Y, Zhou Y, Tan Y, Li J, Xiang Y, Nie X, Zhang J, Liu F, Zhao S, Xie S*, Peng G*. Genome-scale CRISPR screen identifies TMEM41B as a multi-function host factor required for coronavirus replication. PLoS Pathog. 2021 Dec 6;17(12):e1010113. doi: 10.1371/journal.ppat.1010113. Online ahead of print.

2.Liu S, Tao D, Liao Y, Yang Y, Sun S, Zhao Y, Yang P, Tang Y, Chen B, Liu Y, Xie S*, Tang Z*. Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus. ACS Synth Biol. 2021 Oct 15;10(10):2499-2507. doi: 10.1021/acssynbio.1c00103. Epub 2021 Sep 20.

3.Zhang J, Khazalwa EM, Abkallo HM, Zhou Y, Nie X, Ruan J, Zhao C, Wang J, Xu J, Li X, Zhao S, Zuo E*, Steinaa L*, Xie S*. The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research. J Genet Genomics. 2021 May 20;48(5):347-360. doi: 10.1016/j.jgg.2021.03.015. Epub 2021 May 11.

4.Han X, Gao Y, Li G, Xiong Y, Zhao C, Ruan J, Ma Y, Li X, Li C, Zhao S, Xie S*. Enhancing the antibacterial activities of sow milk via site-specific knock-in of a lactoferrin gene in pigs using CRISPR/Cas9 technology. Cell Biosci. 2020 Nov 19;10(1):133. doi: 10.1186/s13578-020-00496-y.

5.Zhao C, Liu H, Xiao T, Wang Z, Nie X, Li X, Qian P, Qin L, Han X, Zhang J, Ruan J, Zhu M, Miao YL, Zuo B, Yang K, Xie S*, Zhao S*. CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication. Nat Commun. 2020 Oct 14;11(1):5178. doi: 10.1038/s41467-020-18936-1.

6.Tao D, Liu J, Nie X, Xu B, Tran-Thi TN, Niu L, Liu X, Ruan J, Lan X, Peng G, Sun L, Ma Y, Li X, Li C, Zhao S, Xie S*. Application of CRISPR-Cas12a Enhanced Fluorescence Assay Coupled with Nucleic Acid Amplification for the Sensitive Detection of African Swine Fever Virus. ACS Synth Biol. 2020 Sep 18;9(9):2339-2350. doi: 10.1021/acssynbio.0c00057. Epub 2020 Aug 31. PMID: 32786346

7.Zhao C, Wang Y, Nie X, Han X, Liu H, Li G, Yang G, Ruan J, Ma Y, Li X, Cheng H, Zhao S, Fang Y*, Xie S*. Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity. Int J Biol Sci. 2019 Oct 3;15(12):2641-2653. doi: 10.7150/ijbs.37152. eCollection 2019.

8.Xie S＃, Zhu Q＃, Qu W＃, Xu Z, Liu X, Li X, Li S, Ma W, Miao Y, Zhang L, Du X, Dong W, Li H, Zhao C, Wang Y, Fang Y, Zhao S. sRNAPrimerDB: comprehensive primer design and search web service for small non-coding RNAs. Bioinformatics. 2019 May 1;35(9):1566-1572. doi: 10.1093/bioinformatics/bty852.

9.Ahmad HI, Ahmad MJ, Asif AR, Adnan M, Iqbal MK, Mehmood K, Muhammad SA, Bhuiyan AA, Elokil A, Du X, Zhao C, Liu X*, Xie S*. A Review of CRISPR-Based Genome Editing: Survival, Evolution and Challenges. Curr Issues Mol Biol. 2018;28:47-68. doi: 10.21775/cimb.028.047. Epub 2018 Feb 11.

10.Zhao C, Zheng X, Qu W, Li G, Li X, Miao YL, Han X, Liu X, Li Z, Ma Y, Shao Q, Li H, Sun F*, Xie S*, Zhao S*. CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif. Int J Biol Sci. 2017 Nov 1;13(12):1470-1478. doi: 10.7150/ijbs.21312. eCollection 2017.

11.Xu J, Yao G, Ru Y, Xie S*. Expression of tamoxifen-inducible CRE recombinase in Lcn5-CreERT2 transgenic mouse caput epididymis. Mol Reprod Dev. 2017 Mar;84(3):257-264. doi: 10.1002/mrd.22772. Epub 2017 Mar 16.

12.Xie S, Shen B, Zhang C, Huang X, Zhang Y. sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One. 2014 Jun 23;9(6):e100448. doi: 10.1371/journal.pone.0100448. eCollection 2014.

13.Xie S＃, Xu J＃, Ma W, Liu Q, Han J, Yao G, Huang X, Zhang Y. Lcn5 promoter directs the region-specific expression of cre recombinase in caput epididymidis of transgenic mice. Biol Reprod. 2013 Mar 21;88(3):71. doi: 10.1095/biolreprod.112.104034. Print 2013 Mar.

14.Xie S＃, Li X＃, Liu T, Cao J, Zhong Q, Zhao S. Discovery of porcine microRNAs in multiple tissues by a Solexa deep sequencing approach. PLoS One. 2011 Jan 25;6(1):e16235. doi: 10.1371/journal.pone.0016235.

15.Xie S, Huang T, Shen Y, Li X, Zhang X, Zhu M, Qin H, Zhao S. Identification and characterization of microRNAs from porcine skeletal muscle. Anim Genet. 2010 Apr;41(2):179-90. doi: 10.1111/j.1365-2052.2009.01991.x. Epub 2009 Nov 26.