王璕 博士 副研究员 硕士生导师

研究方向简介

1. 合成生物学与系统生物工程：综合运用分子生物学、系统生物学等手段，在大肠杆菌、芽胞杆菌等细菌中进行人工生命工程化设计。包括：1）新型生物元件开发、基因线路设计等，将其运用于肿瘤等多种人类疾病的治疗；2）对细菌进行代谢途径工程改造，进行精细化工品的设计与生产。

2. 微生物代谢与调控：细菌中第二信使分子，如c-di-AMP, c-di-GMP的代谢调控及其机理的研究；以第二信使分子为配体的核糖开关调控机理的研究。

研究生招生专业

生物工程、微生物学、生物与医药

代表性研究成果

1.       N MPA^#, Wang X^#*, Jeon HJ^*, Lim HM^*. Deciphering the coupling state-dependent transcription termination in the Escherichia coli galactose operon. Mol Microbiol. 2025;123(1):75-87.

2.       Ni Y^#, Wang Y^#, Shi X, Yu F, Ruan Q, Tian N, He J, Wang X^*. Reducing competition between msd and genomic DNA improves retron editing efficiency. EMBO Rep. 2024;25(12):5316-5330.

3.       Liu J^#, Cui L^#, Shi X, Yan J, Wang Y, Ni Y, He J, Wang X^*. Generation of DNAzyme in bacterial cells by a bacterial retron system. ACS Synth Biol. 2024; 13(1):300-309.

4.       Deng YJ, Chen Z, Chen YP, Wang JP, Xiao RF, Wang X, Liu B, Chen MC, He J. Lipopeptide C17 Fengycin B Exhibits a Novel Antifungal Mechanism by Triggering Metacaspase-Dependent Apoptosis in Fusarium oxysporum. J Agric Food Chem. 2024;72(14):7943-7953.

5.       N MPA, Jeon H, Wang X^*, Lim HM^*. Reporter gene-based qRT-PCR assay for Rho-dependent termination in vivo. Cells. 2023; 12(22):2596.

6.       Wang X^#*, Fang C^#, Wang Y, Shi X, Yu F, Xiong J, Chou SH, He J^*. Systematic comparison and rational design of theophylline riboswitches for effective gene repression. Microbiol Spectr. 2023; 11 (1):e0275222.

7.       Wang X^*, N MPA, Jeon HJ, He J, Lim HM^*. Identification of a Rho-dependent termination site in vivo using synthetic small RNA. Microbiol Spectr. 2023; 11(1):e0395022.

8.       Mao G, Luo X, Ye S, Wang X, He J, Kong J, Dai J, Yin W, Ma Y. Fluorescence and colorimetric analysis of african swine fever virus based on the RPA-assisted CRISPR/Cas12a strategy. Anal Chem. 2023; 95(20):8063-8069.

9.       Jeon HJ, Lee Y, Abhishek MPN, Wang X, Chattoraj DK, Lim HM. sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination. PLoS Genet. 2021; 17(10):e1009878.

10.    Jeon HJ, Kang C, Abhishek MPN, Lee Y, Wang X, Chattoraj DK, Lim HM. Translation initiation control of RNase E-mediated decay of polycistronic gal RNA. Front. Mol. Biosci. 2020; 7:586413.

11.    Wang X, Abishek NMP, Jeon HJ, Lee Y, He J, Adhya S, Lim HM. Processing generates 3’ ends of RNA masking transcription termination events in prokaryotes. Proc Natl Acad Sci USA. 2019; 116 (10): 4440-4445.

12.    Wang X, Cai X, Ma HD, Yin W, Zhu L, Li XF, Lim HM, Chou SH, He J. A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis. Commun Biol. 2019; 2 (1): 151.

13.    Wang X, Jeon HJ, Abishek NMP, He J, Lim HM. Visualization of RNA 3’ ends in Escherichia coli using 3’ RACE combined with primer extension. Bio-protocol. 2018; 8 (5): e2752

14.    Wang X, Z. Li, X. Li, H.L. Qian, X. Cai, X.F. Li and J. He. Poly-beta-hydroxybutyrate metabolism is unrelated to the sporulation and parasporal crystal protein formation in Bacillus thuringiensis. Front Microbiol. 2016; 7:836.

15.    Zhang SM, Li XF, Wang X, Li Z, He J. The two-component signal transduction system YvcPQ regulates the bacterial resistance to bacitracin in Bacillus thuringiensis. Archives of Microbiology. 2016; 198: 773-784.

16.    Wang X, Ji SC, Lim HM.Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene. Proc Natl Acad Sci USA. 2015; 112 (24): 7581-7586.

17.    Zhang S, Hu Y, Fan Q, Wang X, He J. Two-component system YvqEC-dependent bacterial resistance against vancomycin in Bacillus thuringiensis. Antonie van Leeuwenhoek. 2015; 108 (2): 365-376.

18.    Zheng C, Ma Y, Wang X, Xie Y, Ali MK and He J. Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis. Front Microbiol. 2015; 6: 908. 

19.    Wang X, Ji SC, Yun SH, Jeon HJ, Kim SW, Lim HM. Expression of each cistron in the gal operon can be regulated by transcription termination and generation of a galK-Specific mRNA, mK2. J Bacteriol. 2014; 196 (14): 2598-2606.

20.    Ji, SC, Wang X, Yun, SH, Jeon, HJ, Lee HJ, Lim, HM. In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase. PLoS ONE. 2011; 6(3): e17646.

21.    Ji, SC, Wang X, Jeon, HJ, Yun, SH, Lee, HJ, Lim, HM. Quantification of the galactose-operon mRNAs 5 bases different in their 5'-ends. BMB Rep. 2010; 43 (7): 474-479.  

主讲课程

合成生物学、微生物学前沿、生物工程创新创业训练、生物工程综合实验四、生物工程前沿等

教学论文

1. 王璕，何进，郝勃等. 新工科背景下“代谢工程”课程建设的思考. 微生物学通报, 2020, 47(3): 1061-1067. 

2. 王璕，何进，韩文元等.基于前沿热点案例的合成生物学教学模式的探索与实践,生物工程学报,2022,38(7):2639-2648.

3.王璕，徐纬，何进.“微生物学前沿”新课程体系的构建.微生物学通报, 2022, 49(4):1326-1333.