王璕 博士 副研究员 硕士生导师

研究方向简介

1. 合成生物学与系统生物工程：综合运用分子生物学、系统生物学等手段，在大肠杆菌、芽胞杆菌等细菌中进行人工生命工程化设计。包括：1）新型生物元件开发、基因线路设计等，将其运用于肿瘤等多种人类疾病的治疗；2）对细菌进行代谢途径工程改造，进行精细化工品的设计与生产。

2. 微生物代谢与调控：细菌中第二信使分子，如c-di-AMP, c-di-GMP的代谢调控及其机理的研究；以第二信使分子为配体的核糖开关调控机理的研究。

研究生招生专业

生物工程、微生物学、生物与医药

代表性研究成果

1. Wang X, Abishek NMP, Jeon HJ, Lee Y, He J, Adhya S, Lim HM. (2019) Processing generates 3’ ends of RNA masking transcription termination events in prokaryotes. P Natl Acad Sci USA 116 (10): 4440-4445. 

2. Wang X, Cai X, Ma HD, Yin W, Zhu L, Li XF, Lim HM, Chou SH, He J. (2019) A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis. Commun Biol 2 (1): 151. 

3. Wang X, Jeon HJ, Abishek NMP, He J, Lim HM. (2018) Visualization of RNA 3’ ends in Escherichia coli using 3’ RACE combined with primer extension. Bio-protocol 8. 

4. Wang X, Z. Li, X. Li, H.L. Qian, X. Cai, X.F. Li & J. He, (2016) Poly-beta-hydroxybutyrate metabolism is unrelated to the sporulation and parasporal crystal protein formation in Bacillus thuringiensis. Front Microbiol 7:836. 

5. Wang X, Ji SC, Lim HM. (2015) Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene. P Natl Acad Sci USA 112: 7581-7586. 

6. Wang X, Ji SC, Yun SH, Jeon HJ, Kim SW, Lim HM. (2014). Expression of each cistron in the gal operon can be regulated by transcription termination and generation of a galK-specific mRNA, mK2. J Bacteriol 196: 2598-2606. 

7. Ji, SC, Wang X, Yun, SH, Jeon, HJ, Lee HJ, Lim, HM. (2011) In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase. PLoS ONE, 6(3): e17646. 

8. Ji, SC, Wang X, Jeon, HJ, Yun, SH, Lee, HJ, Lim, HM. (2010) Quantification of the galactose-operon mRNAs 5 bases different in their 5'-ends. BMB Rep, 43, 474-479. 

9. Zheng C, Ma Y, Wang X, Xie Y, Ali MK and He J. (2015) Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis. Front Microbiol 6: 908. 

10. Zhang S, Hu Y, Fan Q, Wang X, He J. (2015) Two-component system YvqEC-dependent bacterial resistance against vancomycin in Bacillus thuringiensis. Anton Leeuw Int J G. 108(2): 365-376. 

11. Zhang SM, Li XF, Wang X, Li Z, He J. (2016) The two-component signal transduction system YvcPQ regulates the bacterial resistance to bacitracin in Bacillus thuringiensis. Arch Microbiol, 198:773-784. 

12. Yun, SH, Ji, SC, Jeon, HJ, Wang X, Kim, SW, Bak, G., Lee, Y, Lim, HM. (2012) The CnuK9E H-NS complex antagonizes DNA binding of DicA and leads to temperature-dependent filamentous growth in E. coli. PLoS ONE, 7(9): e45236. 

13. Yun, SH, Ji, SC, Jeon, HJ, Wang X, Lee, Y, Choi, BS, Lim, HM. (2012) A mutational study of Cnu reveals attractive forces between Cnu and H-NS. Mol Cells. 33(2): 211-216. 

14. Jeon HJ, Kang C, Abhishek MPN, Lee Y, Wang X, Chattoraj DK, Lim HM (2020) Translation initiation control of RNase E-mediated decay of polycistronic gal RNA. Front. Mol. Biosci. 7:586413.

15. Jeon HJ, Lee Y, Abhishek MPN, Wang X, Chattoraj DK, Lim HM (2021) sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination. PLoS Genet. 2021 Oct 28;17(10):e1009878.

主讲课程

合成生物学、高级代谢工程、生物工程前沿、生物工程综合实验四

教学论文

1. 王璕，何进，郝勃，何璟. 新工科背景下“代谢工程”课程建设的思考. 微生物学通报, 2020, 47(3): 1061-1067.