史凯祥,博士,副研究员,硕士生导师,农业微生物资源发掘与利用全国重点实验室固定研究人员。以微生物学、分子生物学、生态学为主要研究手段,解析:
1. 微生物对重金属的解毒及调控:
解析了微生物砷氧化的调控(Environ Pollut, 2018)、微生物砷抗性系统的建立(Appl Environ Microbiol, 2018)、微生物砷磷共调控(Res Microbiol, 2019)、微生物砷与抗生素共抗性(World J Microbiol Biotechnol, 2019)以及微生物的砷铬协同抗性(Chemosphere, 2020; Environ Sci Technol, 2021),为砷环境中微生物的适应性以及解毒调控机制奠定了理论基础。
2. 微生物在重金属环境下的相互作用与共进化:
揭示了微生物互作促进根瘤菌与大豆结瘤的机制(Soil Biol Biochem, 2023),阐明了重金属镉环境中微生物互作协同钝化镉的分子机制(Mol Plant Microbe Interact, 2023),为理解重金属环境中微生物的互作迈出了重要的一步。
3. 微生物-植物互作缓解重金属的胁迫:
解析了微生物与砷富集植物蜈蚣草的互作机制(Chemosphere, 2023),揭示了菌剂对促进植物和土壤健康与降低镉含量的积极作用(Environ Res, 2021; J Hazard Mater, 2023),阐明了微生物通过代谢产物调控水稻镉转运及结合蛋白的分子机制(Mol Plant Microbe Interact, 2023),为镉污染修复中的微生物-植物互作奠定了理论基础。
科研项目:
1. 国家自然科学基金面上项目:高砷锑抗性肠杆菌Z1沉淀砷和锑的机制,2023.01-2026.12,主持,在研;
2. 国家自然科学基金青年项目:根癌农杆菌GW4在有氧和无氧条件下砷氧化与铬还原相关性的分子机制,2020.01-2022.12,主持,结题;
3. 自主科技创新基金项目:土壤镉铬复合钝化菌剂的田间实验和机理探究,2022.01-2024.12,主持,在研;
4. 中国博士后科学基金面上资助:固定化肠杆菌Z1结合铁盐对铬与砷的协同去除及分子机制,2020.01-2022.12,主持,结题。
代表性论文:
1. Yuxiao Zhang, Qing Xu, Gejiao Wang, Kaixiang Shi*. Mixed Enterobacter and Klebsiella bacteria enhance soybean biological nitrogen fixation ability when combined with rhizobia inoculation. Soil Biol Biochem, 2023, 184:109100.
2. Xing Wang, Qing Xu, Kang Hu, Gejiao Wang*, Kaixiang Shi*. A coculture of Enterobacter and Comamonas reduces Cd accumulation in rice. Mol Plant Microbe Interact, 2023, 36(2):95-108.
3. Aiting Li, Yingying Lu, Da Zhen, Ziheng Guo, Gejiao Wang, Kaixiang Shi*, Shuijiao Liao*. Enterobacter sp. E1 increased arsenic uptake in Pteris vittata by promoting plant growth and dissolving Fe-bound arsenic. Chemosphere, 2023, 329:138663.
4. Xia Fan, Li Nie, Zhengjun Chen, Yongliang Zheng, Gejiao Wang*, Kaixiang Shi*. Simultaneous removal of nitrogen and arsenite by heterotrophic nitrification and aerobic denitrification bacterium Hydrogenophaga sp. H7. Front Microbiol, 2023, 13:1103913.
5. Shijuan Wu, Zijie Zhou, Lin Zhu, Limin Zhong, Yixuan Dong, Gejiao Wang*, Kaixiang Shi*. Cd immobilization mechanisms in a Pseudomonas strain and its application in soil Cd remediation. J Hazard Mater, 2022, 425:127919.
6. Zijie Zhou, Yixuan Dong, Lin Zhu, Xian Xia, Sikui Li, Gejiao Wang*, Kaixiang Shi*. Effective and stable adsorptive removal of cadmium(II) and lead(II) using selenium nanoparticles modified by microbial SmtA metallothionein. Chemosphere, 2022, 307:135818.
7. Yuxiao Zhang, Qing Xu, Gejiao Wang*, Kaixiang Shi*. Indole-acetic acid promotes ammonia removal through heterotrophic nitrification, aerobic denitrification with mixed Enterobacter sp. Z1 and Klebsiella sp. Z2. Front Microbiol, 2022, 13:929036.
8. Kaixiang Shi, Manohar Radhakrishnan, Xingli Dai, Barry P. Rosen*, Gejiao Wang*. NemA catalyzes trivalent organoarsenicals oxidation and is regulated by the trivalent organoarsenical-selective transcriptional repressor NemR. Environ Sci Technol, 2021, 55(9):6485-6494.
9. Ying Yu#, Kaixiang Shi#, Xuexue Li, Xiong Luo, Mengjie Wang, Lin Li, Gejiao Wang, Mingshun Li*. Reducing cadmium in rice using metallothionein surface-engineered bacteria WH16-1-MT. Environ Res, 2021, 203:111801.
10. Kaixiang Shi, Xingli Dai, Xia Fan, Yuxiao Zhang, Zhengjun Chen, Gejiao Wang*. Simultaneous removal of chromate and arsenite by the immobilized Enterobacter bacterium in combination with chemical reagents. Chemosphere, 2020, 259, 127428.
11. Kaixiang Shi, Qian Wang, Gejiao Wang*. Microbial oxidation of arsenite: Regulation, chemotaxis, phosphate metabolism and energy generation. Front Microbiol, 2020, 11:569282.
12. Kaixiang Shi#, Min Cao#, Chan Li, Jing Huang, Shixue Zheng, Gejiao Wang*. Efflux proteins MacAB confer the resistance to arsenite and penicillin/macrolide-type antibiotics and in Agrobacterium tumefaciens 5A. World J Microbiol Biotechnol, 2019, 35, 115.
13. Zixu Qiao, Jing Huang, Yajing Cao, Kaixiang Shi*, Gejiao Wang*. Genetics and proteomics analyses reveal the roles of PhoB1 and PhoB2 regulators in bacterial responses to arsenite and phosphate. Res Microbiol, 2019, 170, 263-271.
14. Kaixiang Shi, Chan Li, Christopher Rensing, Xingli Dai, Xia Fan, Gejiao Wang*. Efflux transporter ArsK is responsible for bacterial resistance to arsenite, antimonite, trivalent roxarsone and methylarsenite. Appl Environ Microbiol, 2018, 84.
15. Kaixiang Shi, Qian Wang, Xia Fan, Gejiao Wang*. Proteomics and genetic analyses reveal the effects of arsenite oxidation on metabolic pathways and the roles of AioR in Agrobacterium tumefaciens GW4. Environ Pollut, 2018, 235, 700-709.
16. Kaixiang Shi, Xia Fan, Zixu Qiao, Yushan Han, Timothy R McDermott, Qian Wang*, Gejiao Wang*. Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4. Sci Rep, 2017, 7, 43252.