研究方向：

多细胞真核生物基因组中包含大量的非编码调控序列，其通过三维基因组远程互作，对基因的表达具有重要调控作用。本人一直围绕表观三维基因组对基因转录调控作用等科学问题开展研究，期望通过鉴定作物中的顺式调控元件并解析其参与的三维基因组远程互作，解析基因组中非编码序列的功能。在植物中建立了单细胞核snCUT&Tag技术，实现从单细胞水平鉴定细胞类型特异性顺式调控元件。在水稻杂种鉴定了大量沉默子调控序列，并发现沉默子通过与远端功能基因发生远程染色质互作从而调控功能基因的沉默进而调整植株的表型性状。系统鉴定了水稻基因组的相分离相关蛋白，揭示相分离相关蛋白通过反式作用介导非编码序列的远程互作调控基因的转录和作物表型。

未来将围绕水稻基因组解码和合成开展研究。通过建立调控元件的高通量鉴定和编辑技术，结合三维基因组编辑和高通量表型鉴定，建立“调控元件-表型性状”的对应调控关系，深入研究顺式元件及其参与的染色质远程互作在胁迫响应过程的生物学功能。围绕合成基因组学底层技术，从功能元件解码、基因组智能设计、DNA低成本长片段合成、外源长片段DNA递送和重组、合成植株再生等开展全链条攻关，期待为水稻基因组的全基因组从头设计合成提供技术支撑。

教育经历：

(1) 2015-09 至 2021-06, 华中农业大学, 生物化学与分子生物学, 博士

(2) 2011-09 至 2015-06, 华中农业大学, 张之洞班, 园艺学果树专业, 学士

工作经历：

(1) 2025-09 至今, 湖北洪山实验室/华中农业大学生命科学技术学院, 副研究员

(2) 2021-06 至2025-09, 先后在华中农业大学/奥地利科学技术研究所 (Institute of Science and Technology Austria, ISTA) 开展博士后研究

主持科研项目/课题：

(1) 国家自然科学基金委员会青年科学基金项目, 2023-01-01至2025-12-31, 主持

(2) 湖北省农业关键核心技术攻关项目, 2023年, 子课题主持

(3) 中国博士后科学基金会面上项目, 2022-07, 主持

(4) 中国博士后科学基金会特别资助（站中）, 2022-07, 主持

(5) 湖北省博士后创新研究岗位, 2021-09至2023-09, 主持

(6) 国家自然科学基金委员会面上项目, 2023-01-01至2026-12-31, 参与

(7) 国家自然科学基金委员会面上项目, 2021-01-01至2024-12-31, 参与

(8) 国家自然科学基金委员会面上项目, 2018-01-01至2021-12-31, 参与

(9) 国家自然科学基金委员会青年科学基金项目, 2017-01-01至2019-12-31, 参与

发表文章：

第一或共同第一作者文章 (#, 同等贡献; *, 通讯作者)

1. Shiping Luan#, Runxin Gao#, Guoting Chen, Chuanhao Liu, Denggao Li, Zhifei Xue, Ying Zhang, Yue Li, Cheng Ding, Beibei Liu, Yanyan Liu, Jiapei Yan, Mohamed F Foda, Xingwang Li, Weizhi Ouyang*. Haplotype-resolved 3D genome maps reveal RNAPII-mediated allelic regulation in hybrid rice, aBIOTECH, 2026, https://doi.org/10.1016/j.abiote.2026.100080

2. Runxin Gao#, Minrong Guo#, Shiping Luan#, Weizhi Ouyang*, Xingwang Li*. ricePSP: a database of rice phase separation-associated proteins, Genome Biology, 2025, 26:390.

3. Weizhi Ouyang#, Xiwen Zhang#, Minrong Guo, Jing Wang, Xiaoting Wang, Runxin Gao, Meng Ma, Xu Xiang, Shiping Luan, Zhilin Cao, Feng Xing, Jiapei Yan, Guoliang Li*, Xingwang Li*. Haplotype mapping of H3K27me3-associated chromatin interactions defines topological regulation of gene silencing in rice, Cell Reports, 2023, 42: 112350.

4. Weizhi Ouyang* and Xingwang Li*. CUT&Tag for Mapping In Vivo Protein-DNA Interactions in Plants. In: Kaufmann, K., Vandepoele, K. (eds) Plant Gene Regulatory Networks. Methods in Molecular Biology, 2023, 2698:109-117.

5. Weizhi Ouyang* and Xingwang Li*. Mapping Active Gene-Associated Chromatin Loops by ChIA-PET in Rice. In: Kaufmann, K., Vandepoele, K. (eds) Plant Gene Regulatory Networks. Methods in Molecular Biology, 2023, 2698:183-194.

6. Weizhi Ouyang#, Shiping Luan#, Xu Xiang, Minrong Guo, Yan Zhang, Guoliang Li, Xingwang Li*. Profiling plant histone modification at single-cell resolution using snCUT&Tag, Plant Biotechnology Journal, 2022, 20: 420-422. (cover story)

7. Weizhi Ouyang, Xiwen Zhang, Yong Peng, Qing Zhang, Zhilin Cao, Guoliang Li, Xingwang Li*. Rapid and low-input profiling of histone marks in plants using nucleus CUT&Tag, Frontiers in Plant Science, 2021, 12: 634679.

8. Weizhi Ouyang#, Qin Xiao#, Guoliang Li*, Xingwang Li*. Technologies for capturing 3D genome architecture in plants, Trends in Plant Science, 2021, 26: 196-197.

9. Weizhi Ouyang#, Dan Xiong#, Guoliang Li*, Xingwang Li*. Unraveling the 3D genome architecture in plants: present and future, Molecular Plant, 2020, 13: 1676-1693.

10. Weizhi Ouyang#, Zhilin Cao#, Dan Xiong, Guoliang Li*, Xingwang Li*. Decoding the plant genome: From epigenome to 3D organization, Journal of Genetics and Genomics, 2020, 47: 425-434.

11. Sibei Li#, Weizhi Ouyang#, Xiaojin Hou, Liangliang Xie, Chungen Hu, Jinzhi Zhang*. Genome-wide identification, isolation and expression analysis of auxin response factor (ARF) gene family in sweet orange (Citrus sinensis), Frontiers in Plant Science, 2015, 6:119.

参与作者文章：

12. James Walker#, Jingyi Zhang#, Yalin Liu#, Shujuan Xu#, Yiming Yu, Martin Vickers, Weizhi Ouyang, Judit Tálas, Liam Dolan, Keiji Nakajima, Xiaoqi Feng*. Extensive N4 cytosine methylation is essential for Marchantia sperm function, Cell, 2025, 188: 2890-2906.e14.

13. Shaotong Chen, Fuquan Li, Weizhi Ouyang, Shuifu Chen, Sanyang Luo, Jianhong Liu, Gufeng Li, Zhansheng Lin, Yao-guang Liu, Xianrong Xie*. Time-course transcriptome and chromatin accessibility analyses reveal the dynamic transcriptional regulation shaping spikelet hull size, Plant Journal, 2025, 122: e70141.

14. Yu Chang#, Jiahan Liu#, Minrong Guo#, Weizhi Ouyang, Jiapei Yan, Lizhong Xiong*, Xingwang Li*. Drought-responsive dynamics of H3K9ac-marked 3D chromatin interactions are integrated by OsbZIP23-associated super-enhancer-like promoter regions in rice, Genome Biology, 2024, 25: 262.

15. Zhifei Xue, Baibai Gao, Guoting Chen, Jie Liu, Weizhi Ouyang, Mohamed Frahat Foda, Qing Zhang, Xiwen Zhang, Wei Zhang, Mingyue Guo, Xingwang Li*, Bin Yi*. Diurnal oscillations of epigenetic modifications are associated with variation in rhythmic expression of homoeologous genes in Brassica napus, BMC Biology, 2023, 21:241.

16. Wei Zhang, Huanhuan Wang, Yuning Ma, Baibai Gao, Pengpeng Guan, Xingyu Huang, Weizhi Ouyang, Minrong Guo, Guoting Chen, Guoliang Li, Xingwang Li*. Domains Rearranged Methylase 2 maintains DNA methylation at large DNA hypomethylated shores and long-range chromatin interactions in rice, Plant Biotechnology Journal, 2023, 21:2333-2347.

17. Ying Zhang#, Guoting Chen#, Li Deng, Baibai Gao, Jing Yang, Cheng Ding, Qing Zhang, Weizhi Ouyang, Minrong Guo, Wenxia Wang, Beibei Liu, Qinghua Zhang, Wing-Kin Sung, Jiapei Yan*, Guoliang Li*, Xingwang Li*. Integrated 3D genome, epigenome and transcriptome analyses reveal transcriptional coordination of circadian rhythm in rice, Nucleic Acids Research, 2023, 51:9001-9018.

18. Qing Zhang#, Pengpeng Guan#, Lun Zhao#, Meng Ma, Liang Xie, Yue Li, Ruiqin Zheng, Weizhi Ouyang, Shunyao Wang, Hongmeijuan Li, Ying Zhang, Yong Peng, Zhilin Cao, Wei Zhang, Qin Xiao, Yuanling Xiao, Tingdong Fu, Guoliang Li*, Xingwang Li*, Jinxiong Shen*. Asymmetric epigenome maps of subgenomes reveal imbalanced transcription and distinct evolutionary trends in Brassica napus, Molecular Plant, 2021, 14: 604-619.

19. Lun Zhao#, Liang Xie#, Qing Zhang#, Weizhi Ouyang, Li Deng, Pengpeng Guan, Meng Ma, Yue Li, Ying Zhang, Qin Xiao, Jingwen Zhang, Hongmeijuan Li, Shunyao Wang, Jiangwei Man, Zhilin Cao, Qinghua Zhang, Qifa Zhang, Guoliang Li*, Xingwang Li*. Integrative analysis of reference epigenomes in 20 rice varieties, Nat Communications, 2020, 11: 2658.

20. Lun Zhao#, Shuangqi Wang#, Zhilin Cao#, Weizhi Ouyang, Qing Zhang, Liang Xie, Ruiqin Zheng, Minrong Guo, Meng Ma, Zhe Hu, Wing-Kin Sung, Qifa Zhang, Guoliang Li*, Xingwang Li*. Chromatin loops associated with active genes and heterochromatin shape rice genome architecture for transcriptional regulation, Nat Communications, 2019, 10: 3640.

21. Yong Peng#, Dan Xiong#, Lun Zhao, Weizhi Ouyang, Shuangqi Wang, Jun Sun, Qing Zhang, Pengpeng Guan, Liang Xie, Wenqiang Li, Guoliang Li*, Jianbing Yan*, Xingwang Li*. Chromatin interaction maps reveal genetic regulation for quantitative traits in maize, Nat Communications, 2019, 10: 2632.