李英俊 博士

        华中农业大学生命科学技术学院教授，博士生导师，农业微生物资源发掘与利用全国重点实验室固定研究人员。农业农村部饲料和饲料添加剂生产许可专家委员会委员，中国动物疫病预防控制中心兽医与屠宰领域农机装备专家指导组成员，农业农村部饲用抗生素替代技术重点实验室学术委员会特聘顾问，全国饲料工业标准化技术委员会微生物及酶制剂标准化工作组委员，中国饲料工业协会生物饲料技术委员会委员，中国生物工程学会噬菌体技术专业委员会委员，中国畜牧协会生物产业分会理事会理事。担任iMeta青年编委，Engineering microbiology青年执行编辑，Biofeed Science and Technology编委，《基因组学与应用生物学》编委等。荣获江苏省“双创人才”，武汉英才“优秀青年人才”，光谷3551“产业教授”，宿迁英才雄英计划，2018年博士后创新人才支持计划等人才资助。

        专注益生菌工程新理论与新技术的应用基础研究，从益生菌菌株和基因资源出发，解析益生菌的功能与机制，创制满足动植物与人类营养、健康需求的生物制品，形成了系统且畅通的科技创新和产业转化体系。研究成果在Trends Biotechnol，Mol Cell，Nucleic acids Res，Small，Anal Chem，ACS Synth Biol，mLife等国内外期刊发表论文50余篇，撰写英文专著2章，申请发明专利20余项。

目前课题组研究方向：

（1）益生菌与环境互作：益生菌环境适应、动植物互作及其宿主防御机理解析；

（2）益生菌与动植物营养：益生菌生物制造，开发微生态制剂、生物饲料、生物肥料等；

（3）益生菌与人类抗衰：以益生菌为底盘，打造工程菌智能体，开发抗衰老工程益生菌。

招生招聘
欢迎报考微生物学、生物工程和生物与医药三个专业的硕士研究生和博士研究生，同时，课题组还招聘博士后、科研助理1-2名。有意向的同学请联系：
E-mail：yingjun@mail.hzau.edu.cn。

发表论文

1. Li, D., Feng, J., Wang, X., Zhao, E., Liang, Y., Li, K. and Li, Y.* (2026) An Anaerobic Fluorescent Reporter System and CRISPR-Cas12a Enable High-Throughput Metabolic Engineering of Clostridium butyricum. ACS Synth Biol.

2. Song, J., Zhao, M., Hu, M., Wang, X., Chen, Y.*, Liu, Y.* and Li, Y.* (2026) A Rapid and Sensitive Detection Cascade for Bacillus subtilis Vegetative Cells Utilizing Phage-Derived CBD and Lysin. Analytical chemistry.10.1021/acs.analchem.5c07819.

3. Bu, X.Y., Guo, Y.W., Tian, X.Y., Shao, Y., Li, C.Y., Zhang, P., Liang, Y.X. and Li, Y.J.* (2026) A novel two-component system regulates shikimate pathway for thermal adaptation in Thermus thermophilus. Nucleic Acids Research, 54.

4. Liu, Q., Huang, Y., Zhou, R., Ding, H., Nie, Q., Gong, X., Zuo, T., Wang, S., Liang, Y. and Li, Y.* (2026) A Minimal and Portable CRISPR Platform Based on Bifidobacterial Cas9 Enables Genome Editing in E. coli Nissle 1917. ACS Synth Biol.

5. Shao, Y., Zhang, P., Su, M., Wei, J., Liang, Y., Bu, X., Motawaa, M., Li, H.* and Li, Y.* (2026) SSB-mediated enhancement of argonaute activity triggers SOS filamentation in bacteria. Nucleic Acids Research.

6. Song, J., Hu, M., Zhao, M., Luo, S., Chen, Y., Liu, Y.* and Li, Y.*(2026) Characterization of Bacillus subtilis phages PJNB030, PJNB031, and PJNB032 reveals wall teichoic acid as a key receptor determinant. Engineering Microbiology, 6.

7. Bai, G., Li, X., Ding, H., Li, D., Li, K., Zhou, W., Liang, Y. and Li, Y.* (2026) Chitooligosaccharides promote intestinal adhesion of Clostridium butyricum and enhance gut health, immunity, and antioxidant capacity in largemouth bass. Carbohydrate Polymer Technologies and Applications, 13.

8. Hu, Z., Su, M., Liu, Q., Li, Y., Liang, Y., Li, S. and Li, Y.* (2025) Systems metabolic engineering of glutathione biosynthesis in Saccharomyces cerevisiae: Pathway balancing coupled with enzyme screening for high-titer production. Engineering Microbiology, 5.

9. Li, X., Bai, G., Feng, L., Ding, H., Li, K., Zhou, W., Liang, Y. and Li, Y.* (2025) Biosynthetic Nano-Selenium by Bacillus licheniformis Enhances Growth and Health of Largemouth Bass (Micropterus salmoides). J Microbiol Biotechnol, 35, e2508049.

10. Yin, Y.X., Xie, R.H., Yang, Y.C., Su, A.M., Chen, Z., Liu, X.D., Li, Y.G., Li, Y.J. and Liang, Y.X.* (2025) Novel pilot-scale air-lift inner circulation bioreactor for enhanced nitrogen removal from digested swine wastewater via partial nitritation-anammox. J Water Process Eng, 79.

11. Liang, Y., Motawaa, M., Bu, X., Wei, J., Shao, Y. and Li, Y.* (2025) Construction of primary chassis cells with efficient protein expression in Thermus thermophilus. Microb Cell Fact, 24, 163.

12. Wei, J., Motawaa, M. and Li, Y.* (2025) Thermus thermophilus CRISPR Cas6 Heterologous Expression and Purification. Bio-protocol, 8, 793.

13. Wei, J., Shao, Y., Liang, Y., Bu, X., Zhou, W., Liang, Y. and Li, Y.* (2025) A type III-associated Cas6 functions as a negative regulator of type I-B CRISPR-Cas system in Thermus thermophilus. Commun Biol, 8, 793.

14. Jia, X., Dun, Y., Xiang, G., Wang, S., Zhang, H., Zhou, W., Li, Y.* and Liang, Y. *(2025) The Effects of Pretreated and Fermented Corn Stalks on Growth Performance, Nutrient Digestion, Intestinal Structure and Function, and Immune Function in New Zealand Rabbits. Animals, 15.

15. Jia, X., Dun, Y., Zhang, M., Mei, Y., Li, Y.* and Liang, Y.* (2025) Fermented Corn Straw Increases Cellulase Activity, Improves Rumen Fermentation, and Increases Nutrient Digestibility in Yichang White Goats. Fermentation, 11

16. Zhang, H., Sun, J.Z., Xie, A.W., Yang, H.Q., Li, Y.J., Mei, Y.X., Li, J.S., Xiao, L., Liu, Y.Y. and Liang, Y.X. * (2025) Enhancing butyrate synthesis and intestinal epithelial energy supply through mixed probiotic intervention in dextran sulfate sodium-induced colitis. Food Bioscience, 63.

17. Zheng, B., Fu, Q., Lu, C., Liu, X., Zhang, D., Wang, L., Lv, X., Li, Y., Jiang, Y. and Wang, S.* (2025) Moutai distiller’s grains as an alternative ingredient to corn and soybean meal in broiler diets. Journal of Applied Animal Research, 53.

18. Huang, Z., Wei, L., Zhou, Y., Li, Y.* and Chen, Y.* (2024) Guide DNA dephosphorylation-modulated Pyrococcus furiosus Argonaute fluorescence biosensor for the detection of alkaline phosphatase and aflatoxins B1. Biosensors and Bioelectronics, 265, 116692.

19. Qiu, Q., Zhan, Z., Zhou, Y., Zhang, W., Gu, L., Wang, Q., He, J., Liang, Y., Zhou, W.* and Li, Y.* (2024) Effects of Yeast Culture on Laying Performance, Antioxidant Properties, Intestinal Morphology, and Intestinal Flora of Laying Hens. Antioxidants, 13.

20. Yang, Y., Shao, Y., Pei, C., Liu, Y., Zhang, M., Zhu, X., Li, J., Feng, L., Li, G., Li, K., Liang, Y., Li, Y.* (2024) Pangenome analyses of Clostridium butyricum provide insights into its genetic characteristics and industrial application. Genomics, 116, 110855.

21. Wang, X., Hu, X., Pan, S., Zhang, Y., Zhao, J., Jiang, F., Li, Y. *and Chen, Y.* (2024) DNA and ATP synergistically triggered Argonaute-mediated sensor for the ultrasensitive detection of viable Salmonella without DNA extraction and amplification. Sensors and Actuators B: Chemical, 408.

22. Wang, Z., Feng, N., Zhou, Y., Cheng, X., Zhou, C., Ma, A., Wang, Q., Li, Y.* and Chen, Y. *(2024) Mesophilic Argonaute-Mediated Polydisperse Droplet Biosensor for Amplification-Free, One-Pot, and Multiplexed Nucleic Acid Detection Using Deep Learning. Analytical chemistry, 408.

23. Lu, Y., Wen, J., Wang, C., Wang, M., Jiang, F., Miao, L., Xu, M., Li, Y.*, Chen, X.* and Chen, Y.* (2023) Mesophilic Argonaute-Based Single Polystyrene Sphere Aptamer Fluorescence Platform for the Multiplexed and Ultrasensitive Detection of Non-Nucleic Acid Targets. Small, e2308424.

24. Wei, J. and Li, Y.* (2023) CRISPR-based gene editing technology and its application in microbial engineering. Eng Microbiol, 3, 100101.

25. Fan, Z.Y., Mei, Y.X., Xing, J.W., Chen, T., Hu, D., Liu, H., Li, Y.J., Liu, D.R., Liu, Z.F. and Liang, Y.X.* (2023) Loop-mediated isothermal amplification (LAMP)/Cas12a assay for detection of in tomato. Front Bioeng Biotech, 11, 1188176.

26. Liu, Y., Zhang, H., Xie, A., Sun, J., Yang, H., Li, J., Li, Y., Chen, F., Mei, Y.* and Liang, Y.* (2023) Lactobacillus rhamnosus and L. plantarum Combination Treatment Ameliorated Colitis Symptoms in a Mouse Model by Altering Intestinal Microbial Composition and Suppressing Inflammatory Response. Mol Nutr Food Res, 67, e2200340.

27. Qin, Y., Ke, W., Zhou, Y., Zhu, D., Li, Y.* and Hu, Y.* (2023) TtAgo sensor for the sensitive and rapid detection of T4 polynucleotide kinase activity. Sensors and Actuators B: Chemical, 386.

28. Jin, X., Su, M., Liang, Y. and Li, Y.* (2022) Effects of chlorogenic acid on growth, metabolism, antioxidation, immunity, and intestinal flora of crucian carp (Carassius auratus). Frontiers in microbiology, 13, 1084500.

29. Wang, J., Wei, J., Li, H. and Li, Y.* (2022) High‐efficiency genome editing of an extreme thermophile Thermus thermophilus using endogenous type I and type III CRISPR‐Cas systems. mLife, 1, 412-427

30. Zou, Y., Qiu, L., Xie, A., Han, W., Zhang, S., Li, J., Zhao, S., Li, Y., Liang, Y. and Hu, Y.* (2022) Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis. Microb Cell Fact, 21, 173.

31. Qin, Y., Li, Y.* and Hu, Y.* (2022) Emerging Argonaute-based nucleic acid biosensors. Trends Biotechnol, 40, 910-914.

32. Jiang Z., Wei J., Peng N., Li Y.* (2021) Genetic Engineering of a Phage-Based Delivery System for Endogenous III-A CRISPR-Cas System Against Mycobacterium tuberculosis. In: Islam M.T., Molla K.A. (eds) CRISPR-Cas Methods. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1657-4_20

33. Chen Y., Li X., Wang S., Qian P., Li Y.* (2021) CRISPR-Cas9-Mediated Genome Editing in Escherichia coli Bacteriophages. In: Islam M.T., Molla K.A. (eds) CRISPR-Cas Methods. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1657-4_21

34. Wang, X., Wu, B., Sui, X., Zhang, Z., Liu, T., Li, Y., Hu, G., He, M. and Peng, N.* (2021) CRISPR-mediated host genomic DNA damage is efficiently repaired through microhomology-mediated end joining in Zymomonas mobilis. Journal of genetics and genomics = Yi chuan xue bao.

35. Ye, Q., Zhao, X., Liu, J., Zeng, Z., Zhang, Z., Liu, T., Li, Y., Han, W. and Peng, N.* (2020) CRISPR-Associated Factor Csa3b Regulates CRISPR Adaptation and Cmr-Mediated RNA Interference in Sulfolobus islandicus. Frontiers in microbiology, 11, 2038.

36. Wu, P., An, J., Chen, L., Zhu, Q., Li, Y., Mei, Y., Chen, Z. and Liang, Y.* (2020) Differential Analysis of Stress Tolerance and Transcriptome of Probiotic Lacticaseibacillus casei Zhang Produced from Solid-State (SSF-SW) and Liquid-State (LSF-MRS) Fermentations. Microorganisms, 8.

37. Wei, J.W., Peng, N., Liang, Y.X., Li, K.K. and Li, Y.J. *(2020) Phage Therapy: Consider the Past, Embrace the Future. Appl Sci-Basel, 10.

38. Sofos, N., Feng, M., Stella, S., Pape, T., Fuglsang, A., Lin, J., Huang, Q., Li, Y., She, Q.* and Montoya, G.* (2020) Structures of the Cmr-β Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas. Molecular Cell, 79, 741-757.e747.

39. Liu, Z., Sun, M., Liu, J., Liu, T., Ye, Q., Li, Y. and Peng, N.* (2020) A CRISPR-associated factor Csa3a regulates DNA damage repair in Crenarchaeon Sulfolobus islandicus. Nucleic Acids Res, 48, 9681-9693.

40. Jiang, Z., Wei, J., Liang, Y., Peng, N.* and Li, Y.* (2020) Aminoglycoside Antibiotics Inhibit Mycobacteriophage Infection. Antibiotics (Basel), 9.

41. Zhang, Z., Pan, S., Liu, T., Li, Y. and Peng, N.* (2019) Cas4 Nucleases Can Effect Specific Integration of CRISPR Spacers. J Bacteriol, 201.

42. Pan, S., Li, Q., Deng, L., Jiang, S., Jin, X., Peng, N., Liang, Y., She, Q. and Li, Y.* (2019) A seed motif for target RNA capture enables efficient immune defence by a type III-B CRISPR-Cas system. RNA biology, 16, 1166-1178.

43. Li, Y.* and Peng, N.* (2019) Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials: Review and Prospects. Frontiers in microbiology, 10, 2471.

44. Liu, T., Pan, S., Li, Y., Peng, N. and She, Q. (2018) Type III CRISPR-Cas System: Introduction And Its Application for Genetic Manipulations. Curr Issues Mol Biol, 26, 1-14.

45. Peng, N., Han, W., Li, Y., Liang, Y. and She, Q. (2017) Genetic technologies for extremely thermophilic microorganisms of Sulfolobus, the only genetically tractable genus of crenarchaea. Science China. Life sciences, 60, 370-385.

46. Liu, T., Liu, Z., Ye, Q., Pan, S., Wang, X., Li, Y., Peng, W., Liang, Y., She, Q. and Peng, N. (2017) Coupling transcriptional activation of CRISPR-Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus. Nucleic Acids Res, 45, 8978-8992.

47. Li, Y., Zhang, Y., Lin, J., Pan, S., Han, W., Peng, N., Liang, Y.X. and She, Q. (2017) Cmr1 enables efficient RNA and DNA interference of a III-B CRISPR-Cas system by binding to target RNA and crRNA. Nucleic Acids Res, 45, 11305-11314.

48. Han, W.#, Li, Y.#, Deng, L., Feng, M., Peng, W., Hallstrom, S., Zhang, J., Peng, N., Liang, Y.X., White, M.F. et al. (2017) A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction. Nucleic Acids Res, 45, 1983-1993.

49. Li, Y., Pan, S., Zhang, Y., Ren, M., Feng, M., Peng, N., Chen, L., Liang, Y.X. and She, Q. (2016) Harnessing Type I and Type III CRISPR-Cas systems for genome editing. Nucleic Acids Res, 44, e34.

50. Liu, T., Li, Y., Wang, X., Ye, Q., Li, H., Liang, Y., She, Q. and Peng, N. (2015) Transcriptional regulator-mediated activation of adaptation genes triggers CRISPR de novo spacer acquisition. Nucleic Acids Res, 43, 1044-1055.

51. Ao, X., Li, Y., Wang, F., Feng, M., Lin, Y., Zhao, S., Liang, Y. and Peng, N. (2013) The Sulfolobus initiator element is an important contributor to promoter strength. J Bacteriol, 195, 5216-5222

专利

1. 一种用于缓解溃疡性结肠炎的副干酪乳酪杆菌胞外囊泡及其应用，李英俊，丁泓皓，聂庆庆，202510470364.4

2. 一种高产虾青素的红发夫酵母菌株及其选育方法与应用，李英俊，李双全，胡志琦，顿耀豪，夏珂，梁运祥，授权号：ZL202111175715.7

3. 一株副蕈状芽孢杆菌及其在硒氧化中的应用，李英俊，李晓虎，梁运祥，梅余霞，陈振民，顿耀豪，刘洋洋，李金山，202310913836X

4. 一种富含生物纳米硒的发酵饲料添加剂及制备方法与应用，李英俊，李晓虎，李克克，梁运祥，2023107836329

5. 一株高产有机酸的人参芽孢杆菌及其在微生态制剂中的应用，李英俊，丁泓皓，刘洋洋，朱曦，梅余霞，陈振民，梁运祥，授权号：ZL202210734326.1

6. 一种嗜热栖热菌基因编辑质粒及在生产超氧化物歧化酶中的应用，李英俊，王瑾婷，危军伟，授权号：ZL202210825581.7

7. 一种利用内源CRISPR-Cas系统进行原核生物基因组编辑的方法，佘群新，梁运祥，李英俊，潘赛夫，任敏，冯明霞，彭楠，授权号：ZL201510639204.4

8. 一种快速检测植物乳杆菌Liang的分子标记组合、引物组合及其检测方法，杨轶成，梁运祥，刘洋洋，杨惠清，谢澳文，张恒，李金山，李英俊，朱曦，梅余霞，授权号：CN202311693178.4

9. 一株低温乳酸菌及其应用，杨惠清，梁运祥，刘洋洋，杨轶成，谢澳文，张恒，李金山，朱曦，李英俊，梅余霞，申请号：CN202311681463.4

10. 一种生物饲料的发酵罐清洁装置（实用新型），李双全，李斯全，李英俊，田垒，刘斌，童志峰，授权号：ZL202321163527.7

11. 一种氢氧化钙与电子束辐照联合处理固态秸秆的方法，顿耀豪，梁运祥，李英俊，刘洋洋， ZL202310524811.0 授权公告日2024年7月9日

12. 一种发酵秸秆制作方法及其在畜禽养殖中大幅减替玉米中的应用，顿耀豪，梁运祥，李英俊，刘洋洋，2023105192628

13. 一种促进罗伊氏乳杆菌生长的培养基及方法，朱曦，梁运祥，李英俊，梅余霞，刘洋洋，杨惠清，授权号：ZL202211164725.5 授权公告日2024年9月20日

14. 一种富含虾青素的酵母培养物及其制备方法，李双全，李英俊，胡志琦，梁运祥，李斯泉，申请号：CN202210375933.3

15. 一种乳酸菌组合物及其应用，刘洋洋，梁运祥，张恒，孙竞舟，谢澳文，徐小芳，杨惠清，李英俊，梅余霞，授权号：ZL202210482019.9

16. 具有强抗氧化功能的酿酒酵母培养物制备工艺及培养物，梁运祥，李双全，李英俊，李斯泉，申请号：202110426979.9

17. 黄水在反刍动物饲养中的应用，顿耀豪，梁运祥，李英俊，授权号：ZL202110913178.5，2022年5月24日。

18. 一种基于CRISPR-Cas10核酸蛋白复合物的快捷核酸检测定量法，佘群新，梁运祥，李英俊，韩文元，彭楠，申请号：201810002782.0

学术交流

1. 中国微生物学会益生菌专业委员会成立暨学术交流会，2026.04.10-12，合肥，工程菌智能体的理论框架与实践探索

2. 第十一届全国微生物遗传学学术研讨会，2025.08.01-03，乌鲁木齐，MnmA介导tRNA摆动尿苷硫醇化影响嗜热栖热菌温度适应

3. 第九届国际噬菌体科技产业论坛（2024.10.25-10.26），济南，Technological innovation of biological feed and its application in healthy breeding

4. 2023届齐鲁国际讲堂暨第八届国际噬菌体科技产业论坛（2023.11.16-11.17），济南，Bacteriophages and probiotics work together to reduce the use of antibiotics in healthy breeding

5. 2024年生物与化工类学科高质量发展研讨会，2024年12月6-9日，湖南永州，微生物宿主防御系统在分子诊断和工程益生菌中的应用

6. 第八届水产动保配方师大会，2024年10月22-24日，成都，丁酸梭菌培养物—高肽蛋白原料在水产养殖中的应用

7. 第十二届工业微生物大会，2024年9月11-13日，北京，浅谈生物饲料质量与安全控制

8. FIIF 2023饲料行业创新论坛，2023年8月9-11日，中国北京，“饲用微生态制剂技术创新及应用”

9. 第十届中国生物饲料科技大会暨优质畜产品生产技术交流大会，2023年7月30-8月1日，中国沈阳，“生物饲料发酵菌种筛选最新进展”

10. 第二届黄河五岳论坛暨饲料添加剂大会，2023年7月19-20日，中国郑州，“丁酸梭菌及其培养物最新功能研究及应用实例分析”

11. 国家肉鸡技术体系华东技术协作组2023年会，2023年7月1日，中国宿迁，“酵母发酵白酒糟及其在禽类养殖上的应用”

12. 第二届中国益生菌与肠道健康高质量发展论坛，2023年6月12-14日，中国安阳，“丁酸梭菌培养物功能应用”

13. 2022农资头条全球特种肥料大会，2022年6月10-12日，中国南宁，“壳寡糖机理分析及肥料增效应用展望”

14. “生物饲料行业生产与应用技术交流会”，2021年7月3日，泰安，饲料发酵预处理的工艺与菌种选择

15. “畜禽绿色安全养殖暨无抗饲料和替抗养殖技术培训”，2020年10月14日，南京，授课内容：1，乳猪消化道微生态调控原理与应用实例；2，基于发酵饲料与微生态制剂的无抗方案与应用实例

16. “畜禽绿色安全养殖暨无抗饲料和替抗养殖技术培训”，2020年9月23日，西安，乳猪消化道微生态调控原理与应用实例

17. 第四届“中国畜牧生物科技大会”，2020年9月1日，长沙，无抗时代来临，畜牧行业如何应对？

18. “全国农科博士后学术论坛”，2019年12月6日，北京，Molecular mechanism and application of type III-B CRISPR-Cas system

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20. 第二届南湖国际青年科学家论坛，2017年5月17-19，武汉, Molecular mechanism of type III-B CRISPR-Cas system and its application for genetic manipulations.

21. Molecular Biology of Archaea 5，2016年8月1-3，伦敦，英国，Harnessing Type I and Type III CRISPR-Cas systems for genome editing.