Impact Factor:5.64

DOI number:10.3389/fmicb.2021.712473

Journal:2021 Sep 9;12:712473. do

Funded by:National Key Research and Development Program (2016YFD0501302 and 2017YFD0501406), the National Natu

Key Words:Mycoplasma gallisepticum, tylosin, dosage regimen, clinical breakpoint, lung microbiota

Abstract:The aim of this study was to explore the prudent use of tylosin for the treatment of chronic respiratory infectious diseases in chickens caused by Mycoplasma gallisepticum (MG) based on its clinical breakpoint (CBP) and its effect on lung microbiota. The CBP was established based on the wild-type/epidemiological cutoff value (COWT/ECV), pharmacokinetics-pharmacodynamics (PK-PD) cutoff value (COPD), and clinical cutoff value (COCL) of tylosin against MG. The minimum inhibitory concentration (MIC) of tylosin against 111 MG isolates was analyzed and the COWT was 2 μg/ml. M17 with MIC of 2 μg/ml was selected as a representative strain for the PK-PD study. The COPD of tylosin against MG was 1 μg/ml. The dosage regimen formulated by the PK-PD study was 3 days administration of tylosin at a dose of 45.88 mg/kg b.w. with a 24-h interval. Five different MIC MGs were selected for clinical trial, and the COCL of tylosin against MG was 0.5 μg/ml. According to the CLSI decision tree, the CBP of tylosin against MG was set up as 2 μg/ml. The effect of tylosin on lung microbiota of MG-infected chickens was analyzed by 16S rRNA gene sequencing. Significant change of the lung microbiota was observed in the infection group and treatment group based on the principal coordinate analysis and the Venn diagrams of the core and unique OTU. The phyla Firmicutes and Proteobacteria showed difference after MG infection and treatment. This study established the CBP of tylosin against MG. It also provided scientific data for the prudent use of tylosin based on the evaluation of MG infection and tylosin treatment on the lung microbiota.

Co-author:Shuge Wang,Jinli Guo,Yufeng Gu,Jun Li,Lingli Huang,Xu Wang,Yanfei Tao,Zhenli Liu,Zonghui Yuan

First Author:Anxiong Huang

Indexed by:Journal paper

Correspondence Author:Haihong Hao

Document Code:712473

Volume:12

Number of Words:5000

Translation or Not:no

Date of Publication:2021-09-09

Included Journals:SCI

Links to published journals:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458857/