陈小林：男，1981年8月出生，教授，博士生导师，湖北荆门人，农业微生物资源发掘与利用全国重点实验室固定研究人员，华中农业大学植物科学技术学院教师。在植物病理教研室从事教学和科研工作，主讲本科生《微生物学》和《分子植物病理学》，以及研究生《植物病原真菌致病分子机制》。长期从事稻瘟病研究工作，担任中国植物保护学会青年委员会委员和植物病理学报青年编委会委员。

课题组主要聚焦稻瘟菌致病机制，稻瘟菌-水稻互作机制，水稻抗病机制，真菌绿色杀菌剂开发，水稻病害生防技术开发等研究。综合运用功能基因组学，分子遗传学，分子细胞生物学，修饰蛋白组学，表观遗传修饰组学等手段，从翻译后修饰和表观遗传调控的新角度，深入揭示了稻瘟菌侵染结构形成和与水稻互作的分子机理，包括不同翻译后修饰调控附着胞形成关键信号通路和亚细胞结构的分子机制，不同表观遗传修饰调控稻瘟菌附着胞分化和成熟的分子机制，以及稻瘟菌-水稻互作过程中环境适应和免疫逃避的新机制等。为其它非模式病原真菌和非模式寄主植物提供了借鉴，为开发新型杀菌剂提供了候选靶标，提出了真菌病害防控的新策略和新途径，同时也为培育抗病品种提供了理论依据和应用基础。

以通讯作者或第一作者在Ann Rev Phytopathol (2021), Advanced Science (2024), Developmental Cell (2024), The Plant Cell (2014), New Phytologist (2018, 2021, 2022, 2024), Plant Biotechnol Journal (2021), PLOS Pathogens (2020), PLOS Genetics (2017), ACS Appl Mater Interfaces (2019), Environ Microbiol (2020a, 2020b), Mol Plant Pathol (2017, 2019, 2022a, 2022b, 2023)等期刊发表论文38篇，先后主持国家自然科学基金5项（青年基金1项，面上项目4项）。

学习工作经历：

2000.9-2004.6：华中农业大学植物保护系，本科

2004.9-2011.6：中国农业大学植物病理学专业，硕博连读

2011.7-2014.10：中国农业大学作物遗传育种专业，博士后

2014.10-2015.3：北京市农林科学院，科研助理

2015.3-2021.11：华中农业大学，副教授

2019.10-2020.8：英国The Sainsbury Laboratory/John Inners Centre，访问学者

2021.12-至今：华中农业大学，教授

当前主要研究方向：

1）稻瘟菌致病过程的翻译后修饰和表观遗传调控机制

2）水稻抗病过程的翻译后修饰和表观遗传调控机制

3）基于微生态理论的水稻病害绿色防控新技术开发

4）基于计算机辅助设计的新型绿色真菌杀菌剂开发

课题组常年招收博士后，请发送简历至chenxiaolin@mail.hzau.edu.cn

科研项目

1. 国家重点研发计划项目子课题，水稻抗/感稻瘟病相关重要微生物组和代谢化合物鉴定及作用机制，课题编号：2022YFA1304402-03，项目年限：2022/12-2026/11（子课题主持）

2. 国家自然科学基金面上项目，ERF2介导的棕榈酰化修饰调控稻瘟菌附着胞功能的分子机制研究. 课题编号：32272476, 项目年限：2023/1-2026/12（主持）

3. 华中农业大学校自主科技创新基金，稻瘟菌表观遗传和蛋白修饰调控机制，课题编号：2021ZKPY007，项目年限：2021/5-2024/4（主持）

4. 国家自然科学基金面上项目，O-GlcNAc糖基化修饰调控稻瘟菌致病过程的分子机制研究. 课题编号：32072365, 项目年限：2021/1-2024/12（主持）

5. 国家自然科学基金面上项目，SUMO化修饰调控稻瘟菌MAPK信号途径的分子机制研究，课题编号：31871909，项目年限：2019/1-2022/12（主持）

6. 国家自然科学基金青年项目，GPI锚定修饰在稻瘟菌致病过程中的作用和机理研究，课题编号：31601585，项目年限：2017/1-2019/12（主持）

7. 国家自然科学基金面上项目，稻瘟菌酸性pH信号途径的鉴定及其在致病过程中的作用研究，课题编号：31571952，项目年限：2016/1-2019/12（主持）

8. 华中农业大学校自主科技创新基金，稻瘟菌两种糖基化修饰系统的比较研究，课题编号：0900206306，项目年限：2015/1-2017/12（主持）

教学研究与教学改革

华中农业大学襄阳书院项目制实验教学课程建设，2024，主持

华中农业大学实践创新课程提升计划“双百案例”课程，水稻病害研究案例，2022，主持

华中农业大学全英文课程，分子植物病理学，2021，主持

华中农业大学植科院教改项目，新形势下本科“分子植物病理学”课程改革与创新，2019，主持

华中农业大学精品实践课程建设，普通植物病理学实验精品课程，2016，参加

教改论文：陈小林*，董五辈，郑露，杨龙，现代农业背景下植物病理专业“分子植物病理学”课程改革与创新. 教育教学论坛. 2020. 39: 193-194.

主要奖励：

1. 2017年华中农业大学教学质量优秀二等奖

2. 2015年植物科学技术学院教学质量优秀奖

3. 华中农业大学学士学位论文创新奖指导教师(2019,2020)

4. 华中农业大学优秀本科毕业论文指导教师(2020)

5. 华中农业大学本科生SRF项目指导教师(2017,2018,2019,2021)

6. 华中农业大学本科生省创项目指导教师(2021,2024)

7. 华中农业大学本科生国创项目指导教师(2023)

8. 华中农业大学本科生科技创新专项指导教师(2017,2024)

9. 指导本科生获得全国大学生生命科学竞赛二等奖(科学探究类)(2024)

10. 指导研究生获得华中农业大学优秀博士毕业论文(2022)

11. 指导研究生获得华中农业大学优秀硕士毕业论文(2018,2019)

12. 指导研究生获得国家奖学金(2019,2020,2021,2023,2024)

13. 指导研究生获得华中农业大学科研创新奖(2018)

14. 指导研究生获得农业微生物学国家重点实验室年度优秀研究生(2021,2022,2024)

学术论文与著作（*表示通讯作者，#表示共同作者）：

代表性第一或通讯作者文章

1. He R.#, Lv Z.#, Li Y.#, Ren S., Jiaqi Cao, Zhu J., Zhang X., Wu H., Wan L., Xu S., Chen X.L.*, Zhou Z.P.* 2024. tRNA-m1A methylation regulates the infection of Magnaporthe oryzae by supporting ergosterol biosynthesis. Developmental Cell. 22:S1534-5807(24)00485-4.

2. Hu, H.#, He, W.#, Qu, Z., Dong, X., Ren, Z., Qin, M., Liu, H., Zheng, L., Huang, J., Chen, X.L.* 2024. De-nitrosylation coordinates appressorium function for infection of the rice blast fungus. Advanced Science, e2403894. https://doi.org/10.1002/advs.202403894

3. Ren, Z., Dong, X., Guan, L., Yang, L., Liu, C., Cai, X., Hu, H., Lv, Z., Liu, H., Zheng, L., Huang, J., Wilson, R.A., Chen, X.L.* 2024. Sirt5-mediated lysine desuccinylation regulates oxidative stress adaptation in Magnaporthe oryzae during host intracellular infection. New Phytologist, 242(3), 1257–1274.

4. Ren, Z.#, Tang, B.#, Xing, J., Liu, C., Cai, X., Hendy, A., Kamran, M., Liu, H., Zheng, L., Huang, J., Chen, X.L.* 2022. MTA1-mediated RNA m6 A modification regulates autophagy and is required for infection of the rice blast fungus. New Phytologist, 235(1):247-262.

5. Tang, B., Liu, C., Li, Z., Zhang, X., Zhou, S., Wang, G.L., Chen, X.L.*, Liu, W.D.* 2021. Multilayer regulatory landscape during pattern-triggered immunity in rice. Plant Biotechnology Journal, 19(12):2629-2645.

6. Liu W.D.*, Triplett L.*, Chen X.L.* 2021. Emerging roles of posttranslational modifications in plant-pathogenic fungi and bacteria. Annual Review of Phytopathology, 2021(59). 59:99-124.

7. Liu C.Y., Talbot N.J.*, Chen X.L.* 2021. Protein glycosylation during infection by plant pathogenic fungi. New Phytologist. 230(4):1329-1335.

8. Chen X.L., Liu C.Y., Tang B.Z., Ren Z.Y., Wang G.L. and Liu W.D.* 2020. Quantitative proteomics analysis reveals important roles of N-glycosylation on ER quality control system for development and pathogenesis in Magnaporthe oryzae. PLOS Pathogens, 16(2):e1008355.

9. Li S.J.#, Song Z.Y.#, Liu C.Y., Chen X.L.*, Han H.Y.* 2019. Biomimetic mineralization-based CRISPR/Cas9 ribonucleoprotein nanoparticles for gene editing. ACS Applied Materials & Interfaces. 11(51):47762-47770.

10. Liu C.Y.#, Li Z.G.#, Xing J.J., Yang J., Wang Z., Zhang H., Chen D., Peng Y.L., Chen X.L.* 2018. Global analysis of sumoylation function reveals novel insights into development and appressorium-mediated infection of the rice blast fungus. New Phytologist. 219:1031-1047.

11. Li L.W.#, Chen X.L.#, Zhang S.P., Yang J., Chen D., Liu M.X., Zhang H.F., Zheng X.B., Wang P., Peng Y.L.*, Zhang Z.G.* 2017. MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae. PLoS Genetics, 13(5):e1006814.

12. Chen X.L., Shi T., Yang J., Chen D., Xu X.W., Xu J.R., Talbot N.J., Peng Y.L* 2014. N-glycosylation of effector proteins by an alpha-1,3-mannosyltransferase is required to evade host innate immunity by the rice blast fungus. Plant Cell, 26:1360-1376.

其它通讯或第一作者论文(含并列)

1. Chen D., Ju M., Xie J., Chen X.L.*, Peng J.* 2024. Current progress on pathogenicity-related genes in Fusarium oxysporum f. sp. cubense tropical race 4. Phytopathology Research.

2. Hu H., Liu T., Xie X., Li F., Liu C., Jiang J., Li Z., Chen X.L.* 2024. GPI anchoring controls cell wall integrity, immune evasion and surface localization of ChFEM1 for infection of Cochlibolus heterostrophus. Journal of Integrative Agriculture, doi: 10.1016/j.jia.2024.09.033

3. Chen D., Cai X., Xing J., Chen S., Zhao J., Qu Z., Li G., Liu H., Zheng L., Huang J., Chen X.L.* 2023. A lipid droplet associated protein Nem1 regulates appressorium function for infection of Magnaporthe oryzae. aBIOTECH. 4(2), 108-123.

4. Chen D., Kamran M., Chen S., Xing J., Qu Z., Liu C., Ren Z., Cai X, Chen X.L.*, Xu, J.* 2023. Two nucleotide sugar transporters are important for cell wall integrity and full virulence of Magnaporthe oryzae. Molecular Plant Pathology, 24(4), 374-390.

5. Wang W.#*, Cai X.#, Chen X.L.* 2022. Recent Progress of Deubiquitinating Enzymes in Human and Plant Pathogenic Fungi. Biomolecules, 12(10), 1424.

6. Nie Y., Li G., Li J., Zhou X., Zhang Y., Shi Q., Zhou X.*, Li H.*, Chen X.L.*, Li,Y.* 2022. A novel elicitor MoVcpo is necessary for the virulence of Magnaporthe oryzae and triggers rice defense responses. Frontiers in Plant Science, 13, 1018616.

7. Tang B., Zhang Z., Zhao X., Xu Y., Wang L., Chen X.L.*, Wang W.* 2022. Multi-omics analysis reveals a regulatory network of ZmCCT during maize resistance to gibberella stalk rot at the early stage. Frontiers in Plant Science, 13:917493.

8. Liu C.Y., Liu T., Lv ZW, Qin M.Y., Qu Z.G., Zhang Z.W., Li F.Y., Chen D., Zhang X.R., Chen X.L.*, Shen M.* 2022. A calcineurin regulator MoRCN1 is important for asexual development, stress response and plant infection of Magnaporthe oryzae. Frontiers in Plant Science, 13:925645.

9. Cai, X., Xiang, S., He, W., Tang, M., Zhang, S., Chen, D., Zhang, X., Liu, C., Li, G., Xing, J., Li, Y.*, Chen X.L.*, Nie Y.* 2022. Deubiquitinase Ubp3 regulates ribophagy and deubiquitinates Smo1 for appressorium-mediated infection by Magnaporthe oryzae. Molecular Plant Pathology, 23(6):832-844.

10. Chen D.#, Hu H.#, He W., Zhang S., Tang M., Xiang S., Liu C., Cai X., Hendy A., Kamran M, Liu H., Zheng L., Huang J., Chen X.L.*, Xing J.* 2021. Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae. Molecular Plant Pathology, 23(1):133-147.

11. Zhang X.R.#, Zhang Z.H.#, Chen X.L.* 2021. The redox proteome of thiol proteins in the rice blast fungus Magnaporthe oryzae. Frontiers in Microbiology. 12:648894.

12. Zhang X.R., Chen X.L.* 2021. The emerging roles of ubiquitin-like protein Urm1 in eukaryotes. Cellular Signalling. 81 (2021):109946.

13. Li X.H., Zhao H.J., Chen X.L.* 2021. Screening of marine bioactive antimicrobial compounds for plant pathogens. Marine Drugs. 2021, 19:69.

14. Cai X., Yan J., Liu C., Xing J., Ren Z., Hendy A., Zheng L., Huang J., Chen X.L.* 2020. Perilipin LDP1 coordinates lipid droplets formation and utilization for appressorium-mediated infection in Magnaporthe oryzae. Environmental Microbiology, 22(7):2843-2857.

15. Liu C.Y., Xing J.J., Cai X., Hendy A., He W.H., Yang J., Huang J.B., Peng Y.L., Ryder L., Chen X.L.* 2020. GPI7-mediated glycosylphosphatidylinositol (GPI) anchoring regulates appressorial penetration and immune evasion during infection of Magnaporthe oryzae. Environmental Microbiology, 22(7):2581-2595.

16. Cai X.#, Wang Z.#, Hou Y.X., Liu C., Hendy A., Xing J., Chen X.L.* 2020. Systematic characterization of the ubiquitin-specific proteases in Magnaporthe oryzae. Phytopathology Research, 8 (2020).

17. Wang L.Y.#, Cai X.#, Xing J.J., Liu C.Y., Hendy A., Chen X.L.* 2019. URM1-mediated ubiquitin-like modification is required for oxidative stress adaptation during infection of the rice blast fungus. Frontiers in Microbiology, 10:2039.

18. Chen F.F., Ma R.J., Chen X.L.* 2019. Advances of metabolomics in fungal pathogen–plant interactions. Metabolites, 2019(9):169.

19. Hendy A., Xing J.J., Chen X.Y., Chen X.L.* 2019. The farnesyltransferase β-subunit RAM1 regulates localization of RAS proteins and appressorium-mediated infection in Magnaporthe oryzae. Molecular Plant Pathology, 20(9):1264-1278.

20. Chen X.L., Xie X., Liu C.Y., Zeng L.R., Zhou X.P., Luo F., Wang G.L.*, Liu W.D.* 2018. Proteomic analysis of ubiquitinated proteins in rice (Oryza sativa) after treatment with PAMP elicitors. Frontiers in Plant Science, (2018)9:707.

21. Wang Z., Zhang H., Liu C.Y., Xing J.J., Chen X.L.* 2018. A deubiquitinating enzyme Ubp14 is required for development, stress response, nutrient utilization, and pathogenesis of Magnaporthe oryzae. Frontiers in Microbiology, (2018)9:769.

22. Yuan J.L., Wang Z., Xing J.J., Yang Q.Y., Chen X.L.* 2018. Genome-wide Identification and characterization of circular RNAs in the rice blast fungus Magnaporthe oryzae. Scientific Reports, (2018)8:6757.

23. Chen X.L.* 2018. Infection process observation of Magnaporthe oryzae on barley leaves. Bio-protocol, Vol 8, Iss 08. doi: 10.21769/BioProtoc.2833.

24. Chen X.L.#*, Shen M.#, Yang J., Xing Y.F., Chen D., Li Z.G., Zhao W.S., Zhang Y. 2017. Peroxisomal fission is induced during appressorium formation and is required for full virulence of the rice blast fungus. Molecular Plant Pathology, 18(2):222-237.

25. Chen X.L.*, Wang Z., Liu C.Y. 2016. Roles of peroxisomes in the rice blast fungus. BioMed Res Int, 2016:9343417.

26. Chen X.L., Yang J., and Peng Y.L.* 2011. Large-scale insertional mutagenesis in Magnaporthe oryzae by Agrobacterium tumefaciens-mediated transformation. Methods in Molecular Biology, 722:213-224.