王璕 博士 副研究员 硕士生导师

研究方向简介

主要从事合成生物学、基因编辑工具开发与微生物代谢调控研究，聚焦新型Retron基因编辑系统在农业底盘细胞构建与高值产物合成中的应用，致力于以合成生物学手段解决农业生产中的实际问题。

1. 合成生物学与基因编辑工具开发

综合运用分子生物学、系统生物学与合成生物学手段，在枯草芽胞杆菌、大肠杆菌等微生物底盘细胞中进行人工生命系统的工程化设计与应用。当前重点聚焦新型基因编辑工具的开发与农业底盘细胞的构建：

• Retron基因编辑系统：开发基于逆转录子的新型基因编辑工具，实现枯草芽胞杆菌等农业重要菌株的高效、精准基因组编辑；

• 农业底盘细胞工程：以新型基因编辑技术为支撑，构建适用于农业生产的高性能微生物底盘细胞，推动高值农业生物制品的合成与产业化；

• 核酸适配体与生物传感：系统研究核酸适配体在工程菌中的应用策略，开发基于适配体的生物传感与调控模块，拓展合成生物学工具箱。

2. 微生物代谢调控与绿色生物制造

围绕细菌第二信使分子（c-di-AMP、c-di-GMP）的代谢调控网络及其机理展开研究，探索核糖开关等调控元件的功能机制，并在此基础上进行代谢途径的工程化改造：

• 第二信使代谢调控：深入研究c-di-AMP、c-di-GMP等环核苷酸第二信使在细菌生理代谢中的调控机制，解析其合成、降解及信号转导途径；

• 核糖开关调控机理：以第二信使分子为配体，研究核糖开关的结构特征与调控模式，挖掘其在基因表达精细调控中的应用潜力；

• 精细化学品生物合成：对微生物进行代谢途径设计与优化，开展丰原素（Fengycin）等抗菌脂肽类生物农药的可编程设计与绿色高效合成，推动下一代生物农药的研发。

3. 应用导向：从基础研究到农业生物技术转化

• 丰原素下一代可编程生物农药：结合点击化学修饰与合成生物学策略，开发具有靶向性、可编程的高效生物农药制剂；

• 农业高值产物合成：利用工程化农业底盘细胞，推动功能性代谢产物、生物刺激素及绿色农用化学品的微生物合成与产业转化。

研究生招生专业

生物工程、微生物学、生物与医药

代表论文

1. Wang X, Evans NM, Xie YC, He J, Dieckmann T, Liu JW. Towards the last piece of the adenosine aptamer puzzle: A two-site cooperative RNA aptamer. CCS Chem. 2025; 8: 814-825

2. Yu J, Cheng K, Qu S, Wang J, Wang X, Zhao C, Li W. Genome-scale metabolic modeling guided Escherichia coli engineering for de novo biosynthesis of chrysanthemic acid. Adv Sci (Weinh). 2025; 13(2):e12736.

3. N MPA^#, Wang X^#*, Jeon HJ^*, Lim HM^*. Deciphering the coupling state-dependent transcription termination in the Escherichia coli galactose operon. Mol Microbiol. 2025; 123(1):75-87.

4. Jeon HJ, N MPA, Wang X, Lim HM. Rho-dependent termination and RNase E-mediated cleavage: dual pathways for RNA 3' end processing in polycistronic mRNA. J Bacteriol. 2025; 207(3):e0043724.

5. Ni Y^#, Wang Y^#, Shi X, Yu F, Ruan Q, Tian N, He J, Wang X^*. Reducing competition between msd and genomic DNA improves retron editing efficiency. EMBO Rep. 2024; 25(12):5316-5330.

6. Liu J^#, Cui L^#, Shi X, Yan J, Wang Y, Ni Y, He J, Wang X^*. Generation of DNAzyme in bacterial cells by a bacterial retron system. ACS Synth Biol. 2024; 13(1):300-309.

7. Deng YJ, Chen Z, Chen YP, Wang JP, Xiao RF, Wang X, Liu B, Chen MC, He J. Lipopeptide C17 Fengycin B Exhibits a Novel Antifungal Mechanism by Triggering Metacaspase-Dependent Apoptosis in Fusarium oxysporum. J Agric Food Chem. 2024; 72(14):7943-7953.

8. N MPA, Jeon H, Wang X^*, Lim HM^*. Reporter gene-based qRT-PCR assay for Rho-dependent termination in vivo. Cells. 2023; 12(22):2596.

9. Wang X^#*, Fang C^#, Wang Y, Shi X, Yu F, Xiong J, Chou SH, He J^*. Systematic comparison and rational design of theophylline riboswitches for effective gene repression. Microbiol Spectr. 2023; 11 (1):e0275222.

10. Wang X^*, N MPA, Jeon HJ, He J, Lim HM^*. Identification of a Rho-dependent termination site in vivo using synthetic small RNA. Microbiol Spectr. 2023; 11(1):e0395022.

11. Mao G, Luo X, Ye S, Wang X, He J, Kong J, Dai J, Yin W, Ma Y. Fluorescence and colorimetric analysis of african swine fever virus based on the RPA-assisted CRISPR/Cas12a strategy. Anal Chem. 2023; 95(20):8063-8069.

12. Jeon HJ, Lee Y, Abhishek MPN, Wang X, Chattoraj DK, Lim HM. sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination. PLoS Genet. 2021; 17(10):e1009878.

13. Jeon HJ, Kang C, Abhishek MPN, Lee Y, Wang X, Chattoraj DK, Lim HM. Translation initiation control of RNase E-mediated decay of polycistronic gal RNA. Front. Mol. Biosci. 2020; 7:586413.

14. Wang X, Abishek NMP, Jeon HJ, Lee Y, He J, Adhya S, Lim HM. Processing generates 3’ ends of RNA masking transcription termination events in prokaryotes. Proc Natl Acad Sci USA. 2019; 116 (10): 4440-4445.

15. Wang X, Cai X, Ma HD, Yin W, Zhu L, Li XF, Lim HM, Chou SH, He J. A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis. Commun Biol. 2019; 2 (1): 151.

16. Wang X, Jeon HJ, Abishek NMP, He J, Lim HM. Visualization of RNA 3’ ends in Escherichia coli using 3’ RACE combined with primer extension. Bio-protocol. 2018; 8 (5): e2752

17. Wang X, Z. Li, X. Li, H.L. Qian, X. Cai, X.F. Li and J. He. Poly-beta-hydroxybutyrate metabolism is unrelated to the sporulation and parasporal crystal protein formation in Bacillus thuringiensis. Front Microbiol. 2016; 7:836.

18. Zhang SM, Li XF, Wang X, Li Z, He J. The two-component signal transduction system YvcPQ regulates the bacterial resistance to bacitracin in Bacillus thuringiensis. Archives of Microbiology. 2016; 198: 773-784.

19. Wang X, Ji SC, Lim HM.Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene. Proc Natl Acad Sci USA. 2015; 112 (24): 7581-7586.

20. Zhang S, Hu Y, Fan Q, Wang X, He J. Two-component system YvqEC-dependent bacterial resistance against vancomycin in Bacillus thuringiensis. Antonie van Leeuwenhoek. 2015; 108 (2): 365-376.

21. Zheng C, Ma Y, Wang X, Xie Y, Ali MK and He J. Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis. Front Microbiol. 2015; 6: 908.  

22. Wang X, Ji SC, Yun SH, Jeon HJ, Kim SW, Lim HM. Expression of each cistron in the gal operon can be regulated by transcription termination and generation of a galK-Specific mRNA, mK2. J Bacteriol. 2014; 196 (14): 2598-2606.

23. Ji, SC, Wang X, Yun, SH, Jeon, HJ, Lee HJ, Lim, HM. In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase. PLoS ONE. 2011; 6(3): e17646.

24. Ji, SC, Wang X, Jeon, HJ, Yun, SH, Lee, HJ, Lim, HM. Quantification of the galactose-operon mRNAs 5 bases different in their 5'-ends. BMB Rep. 2010; 43 (7): 474-479.  

主讲课程

合成生物学、微生物学前沿、生物分离工程实践等

教学论文

1. 王璕，何进，郝勃等. 新工科背景下“代谢工程”课程建设的思考. 微生物学通报, 2020, 47(3): 1061-1067. 

2. 王璕，何进，韩文元等.基于前沿热点案例的合成生物学教学模式的探索与实践,生物工程学报,2022,38(7):2639-2648.

3.王璕，徐纬，何进.“微生物学前沿”新课程体系的构建.微生物学通报, 2022, 49(4):1326-1333.