通讯地址：430070  湖北省武汉市华中农业大学 果蔬园艺作物种质创新与利用全国重点实验室

Tel: 027-87281543 (O); Email: guoww@mail.hzau.edu.cn

郭文武简介：中共党员，博士、教授、博士生导师。1993年7月本科毕业于华中农业大学园艺系；1993-1998年，华中农业大学攻读硕士/博士学位；1998年6月毕业，获博士学位并留校任教。1999、2003年，分别晋升为副教授、教授。2001-2003年，美国佛罗里达大学柑橘研究与教育中心博士后；2007年，澳大利亚CSIRO园艺部/澳大利亚国立大学高访3个月；2009年，日本鹿儿岛大学高访2个月。

主讲课程：本科生《园艺植物生物技术》《园艺科研案例》，研究生《学术道德与科技写作》《高级果树遗传与育种》

人才团队计划、科研奖励和荣誉称号：入选国家杰出青年科学基金（2012-2015）、教育部人才项目特聘教授（2013-2018）、国家特支计划科技创新领军人才、农业农村部全国农业科研杰出人才（2016-2020）、农业农村部首批神农领军英才（2022）；牵头“园艺植物细胞工程与种质创新”教育部创新团队和湖北省创新群体、“柑橘细胞工程与遗传改良”农业农村部创新团队；曾获中国青年科技奖（2009）、国家科技进步二等奖（2006，第3完成人），“柑橘细胞工程育种技术创新与新品种培育”获湖北省技术发明一等奖（2019，第1完成人）。2020年入选湖北省师德先进个人和华中农业大学第6届师德标兵。

国内外学术兼职：国际SCI刊物《Scientia Horticulturae》共同主编（2014-2022），《Plant Cell Tissue & Organ Culture》《Tree Genetics & Genomes》Associate Editor，《Journal of Integrative Agriculture 》《中国农业科学》《果树学报》等学术期刊编委。国务院学位委员会第7、8届学科评议组（园艺学）成员；中国园艺学会常务理事、分子育种分会副理事长。

科研项目：先后主持国家自然科学基金（面上、杰青、重点、重点国际合作等）、科技部973计划课题、863计划课题、教育部项目（重大项目培育计划、新世纪优秀人才等）、农业部公益性行业科研专项、湖北省自然科学基金（杰青、重点、创新群体）、湖北省重点研发计划项目、瑞典国际科学基金等40余项。

研究兴趣与重点内容 ：从事柑橘细胞工程与遗传改良研究。针对我国柑橘在品种、品质等方面存在的问题，以及常规育种遇到的珠心胚（无性胚）干扰等生殖障碍，借助现代分子标记和组学手段，重点开展细胞工程与遗传改良研究；旨在提高育种效率、缩短育种周期，培育具有无核、优质、不同熟期、多抗广适等性状的新品种（包括砧木），充分发挥“细胞工程”在柑橘遗传改良中的独特作用。重点研究内容：1）细胞工程创新种质与新品种培育：体细胞杂种和胞质杂种创制，离体培养与三倍体创制，同源多倍体发掘，纯系创制，细胞工程新品种培育与应用；2）细胞工程新种质优异性状解析与分子育种：胞质杂种及其遗传群体雄性不育性状解析与基因发掘，人工合成多倍体的体细胞互作遗传、表观遗传与广适应性评价，基于原生质体转染再生的基因编辑技术与分子育种。

发表论著：在PNAS、Plant Phyisology、Plant Biotechnology Journal、Journal of Experimental Botany、Journal of Proteome Research、DNA Research、Metabolomics、BMC Genomics、BMC Plant Biology、Theor Appl Genet、Planta、Plant Science、Plant Cell Reports、Tree Genetics & Genomes、Protoplasma、Plant Cell Tissue & Organ Culture、Euphytica、Scientia Horticulturae、中国农业科学、园艺学报等学术刊物发表第一和通讯（含共同）作者论文100余篇。

部分论著和成果如下（* 通讯作者）

1. Wang N#, Li CC#, Kuang LH, Wu XM, Xie KD, Zhu AD, Xu Q, Larkin RM, Zhou YF, Deng XX, Guo WW* (2022) Pan-mitogenomics reveals the genetic basis of cytonuclear conflicts in citrus hybridization, domestication and diversification. Proc Natl Acad Sci USA 119: e2206076119

2. Zhang M, Tan FQ*, Fan YJ, Wang TT, Song X, Xie KD, Wu XM, Zhang F, Deng XX, Grosser JW, Guo WW* (2022) Acetylome reprograming participates in the establishment of fruit metabolism during polyploidization in citrus. Plant Physiology 190: 2519-2538

3. Feng MQ, Lu MD, Long JM, Yin ZP, Jiang N, Wang PB, Liu Y, Guo WW, Wu XM* (2022) miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus. Journal of Experimental Botany 73: 6170-6185 

4. Jia HH, Xu YT, Yin ZP, Wu XM*, Qing M, Fan YJ, Song X, Xie KD, Xie ZZ, Xu Q, Deng XX, Guo WW (2021) Transcriptomes and DNA methylomes in apomictic cells delineate nucellar embryogenesis initiation in citrus. DNA Research 28: 1-16, DOI: 10.1093/dnares/dsab014

5.  Xia QM, Wang W, Xie KD*, Wu XM, Deng XX, Grosser JW, Guo WW* (2021) Unreduced megagametophyte formation via second division restitution contributes to tetraploid production in interploidy crosses with ‘Orah’ mandarin (Citrus reticulata). Frontiers of Agricultural Science & Engineering 8: 302-313

6.  Wang R, Fang YN, Wu XM*, Qing M, Li CC, Xie KD, Deng XX, Guo WW* (2020) The miR399-CsUBC24 module regulates reproductive development and male fertility in citrus. Plant Physiology 183: 1681-1695 (* co-senior author)

7.  Zhang S, Yin ZP, Wu XM*, Li CC, Xie KD, Deng XX, Grosser JW, Guo WW* (2020) Assembly of Satsuma mandarin mitochondrial genome and identification of cytoplasmic male sterility–specific ORFs in a somatic cybrid of pummelo. Tree Genetics & Genomes 16: 84

8.  Xia QM, Miao LK, Xie KD*, Yin ZP, Wu XM, Chen CL, Grosser JW, Guo WW (2020) Localization of Citrus functional centromeres by combining half-tetrad analysis and CenH3-associated sequence profiling. Plant Cell Reports 39: 1609–1622

9.  Tan FQ, Zhang M, Xie KD, Fan YJ, Song X, Wang R, Wu XM, Zhang HY, Guo WW* (2019) Polyploidy remodels fruit metabolism by modifying carbon source utilization and metabolic flux in Ponkan mandarin. Plant Science 289:110276

10.  Long JM, Liu CY, Feng MQ, Liu Y, Wu XM*, Guo WW* (2018) miR156-SPL modules regulate induction of somatic embryogenesis in citrus callus. Journal of Experimental Botany 69: 2979-2993

11.  Tan FQ, Tu H, Wang R, Wu XM, Xie KD, Chen JJ, Zhang HY, Xu J, Guo WW* (2017) Metabolic adaptation following genome doubling in citrus doubled diploids revealed by non-targeted metabolomics. Metabolomics 13: 143

12.  Long JM, Liu Z, Wu XM*, Fang YN, Jia HH, Xie ZZ, Deng XX, Guo WW* (2016) Genome-scale mRNA and small RNA transcriptomic insights into initiation of citrus apomixis. Journal of Experimental Botany 67: 5743–5756

13.  Fang YN, Zheng BB, Wang L, Yang W, Wu XM, Xu Q, Guo WW* (2016) High-throughput sequencing and degradome analysis reveal altered expression of miRNAs and their targets in a male-sterile cybrid pummelo (Citrus grandis). BMC Genomics 17:591

14.  Guo WW*, Liang WJ, Xie KD, Xia QM, Fu J, Guo DY, Xie ZZ, Wu XM, Xu Q, Yi HL, Deng XX (2016) Exploitation of polyploids from 39 citrus seedling populations. Acta Horticulturae 1135: 11-16

15.  Guo WW*, Xie KD, Zheng BB, Wang SM, Fang YN, Xu Q, Deng XX (2016) Molecular marker assisted cell engineering and citrus improvement. Acta Horticulturae 1127: 265-269

16.  Tan FQ, Tu H, Liang WJ, Long JM, Wu XM, Zhang HY, Guo WW* (2015) Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement. BMC Plant Biology 15: 89

17.  Wang SM, Lan H, Cao HB, Xu Q, Chen CL, Deng XX, Guo WW* (2015) Recovery and characterization of homozygous lines from two sweet orange cultivars via anther culture. Plant Cell Tissue & Organ Culture 123:633-644

18.  Wu XM, Kou SJ, Liu YL, Fang YN, Xu Q, Guo WW* (2015) Genome-wide analysis of small RNAs in non-embryogenic and embryogenic tissues of citrus: microRNA and siRNA mediated transcript cleavage involved in somatic embryogenesis. Plant Biotechnology Journal 13: 383-394

19.  Xie KD, Xia QM, Wang XP, Liang WJ, Wu XM, Grosser JW, Guo WW* (2015) Cytogenetic and SSR marker evidence of mixed disomic, tetrasomic and intermediate inheritance in a citrus allotetraploid somatic hybrid between ‘Nova’ tangelo and ‘HB’ pummelo. Tree Genetics & Genomes 11: 112

20.  Fang YN, Qiu WM, Wang Y, Wu XM, Xu Q, Guo WW* (2014) Identification of differentially expressed microRNAs from a male sterile Ponkan mandarin (Citrus reticulata Blanco) and its fertile wild type by small RNA and degradome sequencing. Tree Genetics & Genomes 10: 1567-1581

21.  Xiao SX, Biswas MK, Li MY, Deng XX, Xu Q, Guo WW* (2014) Production and molecular characterization of diploid and tetraploid somatic cybrid plants between male sterile Satsuma mandarin and seedy sweet orange cultivars. Plant Cell Tissue & Organ Culture 116: 81-88

22.  Xie KD, Wang XP, Biswas MK, Liang WJ, Xu Q, Grosser JW, Guo WW* (2014) 2n megagametophyte formed via SDR contributes to tetraploidization in polyembryonic ‘Nadorcott’ tangor crossed by citrus allotetraploids. Plant Cell Reports 33:1641–1650

23.  Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW* (2014) iTRAQ based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. Journal of Proteome Research 13: 2998-3015

24.  Ahmad T, Sablok G, Tatarinova T, Xu Q, Deng XX, Guo WW* (2013) Evaluation of codon biology in Citrus and Poncirus trifoliata based on genomic features and frame corrected expressed sequence tags. DNA Research 20: 135-150

25.  Guo WW*, Xiao SX, Deng XX (2013) Somatic cybrid production via protoplast fusion for citrus improvement. Scientia Horticulturae 163: 20-26

26.  Ge XX, Chai LJ, Liu Z, Wu XM, Deng XX, Guo WW* (2012) Transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of Valencia sweet orange by SSH based microarray. Planta 236: 1107-1124

27.  Kou SJ, Wu XM, Liu Z, Liu YL, Xu Q, Guo WW* (2012) Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues. Plant Cell Reports 31: 2151-2163

28.  Qiu WM, Zhu AD, Wang Y, Ge XX, Chai LJ, Deng XX, Guo WW* (2012) Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA microarray. BMC Genomics 13: 397

29.  Xu SX, Cai XD, Tan B, Li DL, Guo WW* (2011) Effect of ploidy increase on transgene expression: example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene. Protoplasma 248: 531-540

30.  Wu XM, Liu MY, Ge XX, Xu Q, Guo WW* (2011) Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis ofValenciasweet orange. Planta 233: 495-505

31.  Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW* (2009) Highly efficient transformation of GFP and KNU genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus. Tree Genetics & Genomes 5: 529-537

32.  Cai XD, Fu J, Deng XX, Guo WW* (2007) Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma and two seedy Citrus cultivars. Plant Cell Tissue & Organ Culture 90: 275-283

33.  Guo WW*, Grosser JW* (2005) Somatic hybrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Science 168: 1541-1545

34.  Guo WW, Duan YX, Olivares-Fuster O, Wu ZC, Arias CR, Burns JK, Grosser JW (2005) Protoplast transformation and regeneration of transgenic Valencia sweet orange plants containing a juice quality related pectin methylesterase gene. Plant Cell Reports 24: 482-486

35.  Guo WW, Prasad D, Cheng YJ, Serrano P, Deng XX, Grosser JW (2004) Targeted cybridization in Citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Reports 22: 752-758

36.  Guo WW, Cheng YJ, Deng XX (2002) Regeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata. Plant Cell Reports 20: 829-834

37.  Guo WW, Deng XX (1999) Intertribal hexaploid hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor Appl Genet 98: 581-585

38.  Guo WW, Deng XX (1998) Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Reports 18: 297-300

39. 谢善鹏，解凯东，夏强明，周锐，张成磊，郑浩，伍小萌，郭文武^*（2022）柑橘6个地方品种资源四倍体高效发掘及分子鉴定。果树学报 39：1-9 

40. 解凯东，伍小萌，方燕妮，王蓉，谢宗周，邓秀新，郭文武*（2021）无核柚新品种‘华柚3号’。园艺学报 48(S2): 2815-2816 

41. 解凯东，方燕妮，伍小萌，谢宗周，邓秀新，郭文武*（2020）无核柚新品种‘华柚2号’。园艺学报 47(S2)：2945-2946

42.  解凯东，彭珺，袁东亚，强瑞瑞，谢善鹏，周锐，夏强明，伍小萌，柯甫志，刘高平，Grosser JW，郭文武*（2020）以本地早橘和槾橘为母本倍性杂交创制柑橘三倍体。中国农业科学 53: 4961-4968

43.  周锐，解凯东，王伟，彭珺，谢善鹏，胡益波，伍小萌，郭文武*（2020）依据多倍体形态特征快速高效发掘柑橘四倍体。园艺学报 47: 2451-2458

44.   宋鑫，谭丰全，张苗，蔡元康，郭大勇，解凯东，伍小萌，郭文武*（2019）‘纽荷尔’脐橙与‘尤力克’柠檬种间体细胞杂种的代谢特征分析。园艺学报 46：37-46

45.   夏强明，彭珺，解凯东，伍小萌，徐强，郭文武*（2019）以雄性不育胞质杂种‘华柚2 号’为母本创制柚有性群体。果树学报 36: 961-967

46.   郭文武，叶俊丽，邓秀新*（2019）新中国果树科学研究70年—柑橘。果树学报 36: 1264-1272

47.   梁武军，解凯东，郭大勇，谢宗周，徐强，伊华林，郭文武*（2014）柑橘10个品种实生后代多倍体的发掘与SSR鉴定。园艺学报 41：409-416

48.   解凯东，王晓培，王惠芹，梁武军，谢宗周，郭大勇，伊华林，邓秀新，Grosser JW，郭文武*（2014）以柑橘多胚性二倍体母本倍性杂交培育三倍体。园艺学报 41：613-620

49.   解凯东，王惠芹，王晓培，梁武军，谢宗周，伊华林，邓秀新，Grosser JW，郭文武*（2013）单胚性二倍体为母本与异源四倍体杂交大规模创制柑橘三倍体。中国农业科学 46：4550-4557

50.   农业部植物新品种权（2015）。品种权名：华柚2号；品种权号：CNA20101034.2；品种权人：华中农业大学；培育人：郭文武、程运江、谢宗周、伊华林、邓秀新

51. 农业部植物新品种权（2019）。品种权名：华柚3号；授权号：CNA20191003907；品种权人：华中农业大学；培育人：郭文武、邓秀新、解凯东、谢宗周、蔡小东、李超超、伍小萌

52. 农业部植物新品种权（2019）。品种权名：华柑3号；授权号：CNA20191005407；品种权人：华中农业大学；培育人：郭文武、解凯东、谭丰全、张苗、谢宗周、伍小萌、邓秀新

53.   授权国家发明专利（2016）。发明名称：一种早期筛选柑橘胞质杂种的方法；专利号：ZL 201310221676.9；专利权人：华中农业大学；发明人：郭文武、肖诗鑫

54.   授权国家发明专利（2021）。发明名称：柑橘线粒体InDel分子标记及其应用；专利号：ZL201911042998.0；专利权人：华中农业大学；发明人：郭文武、李超超、张帅、解凯东、伍小萌、徐强、邓秀新

55.   湖北省技术发明一等奖（2019）。成果名称：柑橘细胞工程育种技术创新与新品种培育；成果编号：2019F-027-1-011-001；完成人：郭文武、邓秀新、宋文化、伊华林、谢宗周、解凯东